Quick-RNA Whole Blood

R1201


Quick-RNA Whole Blood

R1201

Cat # Name Size Price Quantity
R1201 Quick-RNA Whole Blood 50 preps $292.00
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Documents


Isolate RNA and small/miRNA from whole blood.

Highlights


  • Superior Yields: Recover total RNA (including small/micro RNAs) without sample loss.
  • Protection: Worry-free blood sample storage at ambient temperatures for up to 30 days.
  • High-Quality: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-PCR, etc. DNase I Included.

Description


The Quick-RNA Whole Blood kit utilizes DNA/RNA Shield™, a unique preservation and lysis technology, to enable rapid isolation of total RNA from whole or partitioned blood or a cell pellet (after red blood cell lysis). The procedure uses Zymo-Spin column technology in which the sample is pre-filtered on the Zymo-Spin IIICG Column and then purified and concentrated on the Zymo-Spin IC Column. RNA is eluted into ≥ 6 µl of RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.

Technical Specifications


Equipment Microcentrifuge, vortex
Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Up to 1 ml mammalian whole-blood, plasma, or serum. Also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted sample from PAXgene™ Blood RNA Tube, etc.) and nucleated blood.
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

Citations


RNA was extracted from canine blood using the Quick-RNA Whole Blood kit and used for PCR amplification for cloning canine TREM-1. TREM-1 expression was shown to be an amplifier or pro-inflammatory responses and has potential to be a biomarker for infection and pneumonia.

Li, J et al. Expression and function of triggering receptor expressed on myeloid cells-1 (TREM-1) on canine neutrophils. Developmental and Comparative Immunology. 2011.

The Quick-RNA Whole Blood kit was used to purify 200 ul human blood and RNA was used to generate cDNA for cloning. Data suggests that the SNPs of aminopeptidase ERAP1 can encode for different normal, hypo-, or hyper- functional activities.

Reeves, E et al. Naturally Occurring ERAP1 Haplotypes Encode Functionally Distinct Alleles with Fine Substrate Specificity. The Journal of Immunology. 2013.

HCV RNA was extracted from sera of human patients using the Quick-RNA Whole Blood kit and used for amplification and Roche 454 pyrosequencing. Results reveal that more genotype 1a isolates were associated with resistance to protease inhibitors.

Margeridon-Thermet, S et al. Similar Prevalence of Low-Abundance Drug-Resistant Variants in Treatment-Naïve Patients with Genotype 1a and 1b Hepatitis C Virus Infections as Determined by Ultradeep Pyrosequencing. PLoS ONE. 2014

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Kit Components