Select-a-Size DNA Clean & Concentrator Kit

D4080


Select-a-Size DNA Clean & Concentrator Kit

D4080

Cat # Name Size Price Quantity
D4080 Select-a-Size DNA Clean & Concentrator Kit 25 Preps $58.00
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Documents


Simple size selection for your DNA sample

Highlights


  • Quick and easy 7 minute spin-column procedure to select for ≥ 300 bp, ≥ 200 bp, ≥ 150 bp, ≥ 100 bp, ≥ 50 bp DNA fragments or perform a double size selection.
  • Clean and concentrate DNA from enzymatic reactions and library preparations in as little as 10 µl of nuclease free water.
  • Eluted DNA is well suited for use in next generation sequencing, PCR, DNA ligation, endonuclease digestion, etc.

Description


The Select-a-Size DNA Clean & Concentrator Kit (Select-a-Size DCC) is a size selection DNA kit that provides the quickest and easiest method for purifying a desired range of DNA fragments sizes from library preps, PCR, endonuclease digestions, ligations, etc. Simply adjust the binding conditions for the desired cutoff, bind, wash, and elute. Selectively recover ≥ 300 bp, ≥ 200 bp, ≥ 150 bp, ≥ 100 bp, ≥ 50 bp DNA fragments or perform a double size selection. Unique Zymo-Spin column technology yields high-quality DNA in just minutes that is suitable for next generation sequencing, PCR, and other downstream applications. The entire purification procedure can be performed in as little as 7 minutes for 2 preps or 20 minutes for 24 samples. (See figures below).

Technical Specifications


Applicable For Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions.
Elution Volume ≥ 10 µl of DNA Elution Buffer
Equipment Microcentrifuge
Purity A260/A280 > 1.8, A260/A230 > 1.8
Sample Source Purified or crude DNA samples.
Size Range ~50 bp to 23 kb. 50 bp, 100 bp, 150 bp, 200 bp, 300 bp separation points. Double size selection: > 700 bp.
Yield Up to 3 µg DNA

Product FAQ


Q1: Can the Select-a-Size kit bind genomic DNA?

Q2: Can the Select-a-Size DCC be used for clean-up of ssDNA?

Q3: What is the difference between Select-a-Size MagBeads and regular MagBinding Beads?

Reviews


This kit worked incredibly well. I was pleased with the purity and concentration of the product.

- author -b

The kit worked great! Both my 260/230 and 260/280 ratios improved. I'm sold.

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First of all, let me introduce myself - I have more then 10 years bench experience in current filed and deal with DNA in my research projects. Downstream of DNA usage is injection in animals and for this reason we need to have pure sample with (sometimes) high concentration. Usually I have been using phenol/chloroform cycle to concentrate or even clean samples (with combination with different one) but outcome not always good. Using this device/protocol makes everything simple and consistent. THANKS!

- Author

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Kit Components