Select-a-Size DNA Clean & Concentrator Kit
- Quick and easy 7 minute spin-column procedure to select for ≥ 300 bp, ≥ 200 bp, ≥ 150 bp, ≥ 100 bp, ≥ 50 bp DNA fragments or perform a double size selection.
- Clean and concentrate DNA from enzymatic reactions and library preparations in as little as 10 µl of nuclease free water.
- Eluted DNA is well suited for use in next generation sequencing, PCR, DNA ligation, endonuclease digestion, etc.
|Applicable For||Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions.|
|Elution Volume||≥ 10 µl of DNA Elution Buffer|
|Purity||A260/A280 > 1.8, A260/A230 > 1.8|
|Sample Source||Purified or crude DNA samples.|
|Size Range||~50 bp to 23 kb. 50 bp, 100 bp, 150 bp, 200 bp, 300 bp separation points. Double size selection: > 700 bp.|
|Yield||Up to 3 µg DNA|
Q1: Can the Select-a-Size kit bind genomic DNA?
Q2: Can the Select-a-Size DCC be used for clean-up of ssDNA?
Q3: What is the difference between Select-a-Size MagBeads and regular MagBinding Beads?
This kit worked incredibly well. I was pleased with the purity and concentration of the product.
- author -b
The kit worked great! Both my 260/230 and 260/280 ratios improved. I'm sold.
First of all, let me introduce myself - I have more then 10 years bench experience in current filed and deal with DNA in my research projects. Downstream of DNA usage is injection in animals and for this reason we need to have pure sample with (sometimes) high concentration. Usually I have been using phenol/chloroform cycle to concentrate or even clean samples (with combination with different one) but outcome not always good. Using this device/protocol makes everything simple and consistent. THANKS!