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ZR BAC DNA Miniprep Kit
D4048 / D4049
ZR BAC DNA Miniprep Kit
- High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
- Unique column design: Zero buffer retention and low elution volume (10 µl)
The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
|Applicable For||Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.|
|Elution Volume||≥ 10 µl|
|Processing Time||15 min|
|Purity||Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0|
|Size Range||Up to ~200 kb|
|Yield||Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.|
Q1: I accidently left my P3 Buffer at room temperature, is it still okay to use?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
|D3004-4-16||DNA Elution Buffer||16 ml||$19.80|
|D3004-4-1||DNA Elution Buffer||1 ml||$12.10|
|D4036-3-60||Endo-Wash Buffer||60 ml||$37.40|
|D4036-3-15||Endo-Wash Buffer||15 ml||$11.00|
|D4036-3-30||Endo-Wash Buffer||30 ml||$19.80|
|D4036-3-6||Endo-Wash Buffer||6 ml||$11.00|
|D4027-1-10||Buffer P1 (Red)||10 ml||$13.20|
|D4027-1-160||Buffer P1 (Red)||160 ml||$28.60|
|D4027-1-80||Buffer P1 (Red)||80 ml||$23.10|
|D4027-2-10||Buffer P2 (Green)||10 ml||$13.20|
|D4027-1-20||Buffer P1 (Red)||20 ml||$16.50|
|D4027-4-6||Plasmid Wash Buffer||6 ml||$11.00|
|D4027-4-48||Plasmid Wash Buffer||48 ml||$53.90|
|D4027-4-24||Plasmid Wash Buffer||24 ml||$38.50|
|D4027-3-50||Buffer P3 (Yellow)||50 ml||$19.80|
|D4027-4-12||Plasmid Wash Buffer||12 ml||$19.80|
|D4027-3-220||Buffer P3 (Yellow)||220 ml||$48.40|
|D4027-3-440||Buffer P3 (Yellow)||440 ml||$88.00|
|D4027-2-80||Buffer P2 (Green)||80 ml||$23.10|
|D4027-3-12||Buffer P3 (Yellow)||12 ml||$15.40|
|D4027-2-20||Buffer P2 (Green)||20 ml||$16.50|
|D4027-2-160||Buffer P2 (Green)||160 ml||$28.60|
|D3004-4-4||DNA Elution Buffer||4 ml||$11.00|
|C1001-50||Collection Tubes||50 Pack||$16.50|
|C1002-25||Zymo-Spin IC-XL||25 Pack||$37.40|
|C1002-50||Zymo-Spin IC-XL||50 Pack||$72.60|