ZR DNA Sequencing Clean-Up Kit

D4050 / D4051


ZR DNA Sequencing Clean-Up Kit

D4050 / D4051

Cat # Name Size Price Quantity
D4050 ZR DNA Sequencing Clean-Up Kit 50 Preps $99.00
+ -
D4051 ZR DNA Sequencing Clean-Up Kit 200 Preps $298.00
+ -

Documents


Reusable and simple purification kit for preparing sequence-ready DNA.

Highlights


  • Complete elimination of "dye blobs" for high quality Phred scores and long read lengths
  • Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps
  • Reusable!

Description


The ZR DNA Sequencing Clean-Up Kits provide a simple and rapid (2 & 10 minute) method for removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data, including dye peaks or "dye blobs" which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide.

Technical Specifications


Elution Volume 6 µl - 20 µl
Equipment Microcentrifuge
Sample Source Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension products
Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
Yield Up to 5 µg DNA

Citations


Kit Components