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Highlights
- High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
- Unique column design: Zero buffer retention and low (30 µl) elution volume.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
Highlights
- High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
- Unique column design: Zero buffer retention and low (30 µl) elution volume.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D3004-4-16 | DNA Elution Buffer | 16 ml | $21.00 | |
| D4036-3-60 | Endo-Wash Buffer | 60 ml | $39.70 | |
| D4036-3-30 | Endo-Wash Buffer | 30 ml | $21.00 | |
| D3004-4-4 | DNA Elution Buffer | 4 ml | $11.60 | |
| D4027-1-160 | Buffer P1 (Red) | 160 ml | $30.40 | |
| D4027-1-20 | Buffer P1 (Red) | 20 ml | $17.50 | |
| D4027-1-80 | Buffer P1 (Red) | 80 ml | $24.50 | |
| D4027-2-160 | Buffer P2 (Green) | 160 ml | $30.40 | |
| D4027-2-20 | Buffer P2 (Green) | 20 ml | $17.50 | |
| D4027-2-80 | Buffer P2 (Green) | 80 ml | $24.50 | |
| D4027-3-220 | Buffer P3 (Yellow) | 220 ml | $51.40 | |
| D4027-3-50 | Buffer P3 (Yellow) | 50 ml | $21.00 | |
| D4027-4-24 | Plasmid Wash Buffer | 24 ml | $40.90 | |
| D4027-4-48 | Plasmid Wash Buffer | 48 ml | $57.20 | |
| D4027-4-6 | Plasmid Wash Buffer | 6 ml | $11.60 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.50 | |
| C1019-50 | Zymo-Spin IIN Columns | 50 Pack | $63.00 | |
Description
Performance
Technical Specifications
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Endotoxin Levels | < 50 EU/µg |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
Resources
Documents
FAQ
The ZymoPURE Miniprep kit utilizes a modified alkaline lysis system in conjunction with a novel binding system. This results in higher yields, higher concentrations, and lower endotoxin levels when compared to the ZR Plasmid Miniprep-Classic.
No, we recommend using our ZR BAC DNA Miniprep Kit (D4048) or ZymoPURE Miniprep Kit for isolating large constructs.
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Yes, but we recommend performing a dry spin in the microcentrifuge after the last wash step, prior to the elution step.
Plasmid Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Zyppy Wash Buffer, or 80% ethanol.
≤ 50 Endotoxin Units/µg of plasmid DNA.
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
Citations
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