ZR small-RNA PAGE Recovery Kit
- Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- Compatible with up to 25% (w/v) polyacrylamide.
|Equipment||Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer|
|Purity||RNA is ready for all subsequent analysis and molecular manipulation|
|Sample Source||RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar).|
|Size Range||RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery)|
|Yield||10 µg RNA (binding capacity), ≥6 µl (elution volume)|
Q1: What is the recovery rate?
Q2: Can the kit be used to recover DNA fragments run on a PAGE gel?
Q3: Can the kit be used to purify DNA/RNA fragments > 200 nucleotides run on a PAGE gel?
Q4: Is the kit compatible with TBE-Urea PAGE?
Q5: What is the maximum amount of gel that can be processed per prep?
Q6: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Uncleaved RNA transcripts were extracted from denaturing (8.3M ura) 10% polyacrylamide gel using the ZR small-RNA PAGE Recovery kit. This facilitates the generation of ribozyme-based control devices for gene regulatory activities.Liang JC, et al. A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity. Nucleic Acids Research. 2012.
The ZR Small-RNA PAGE Recovery kit was used to gel purify short RNAs (50-250 bp) from isolated total RNA on a 15% TBE Urea gel for downstream quantitative PCR.Byun, JS et al. ELL facilitates RNA polymerase II pause site entry and release. Nature Communications. 2012.
In vitro transcribed short hairpin RNA constructs were recovered from 20% denaturing polyacrylamide gels using the ZR small-RNA PAGE Recovery kit. This was used to show that influenza A virus panhandle structure could bind to retinoic acid-inducible gene I (RIG-I) and affect type I interferon production.Liu, GQ et al. Influenza A Virus Panhandle Structure Is Directly Involved in RIG-I Activation and Interferon Induction. Journal of Virology. 2015.
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