ZR small-RNA PAGE Recovery Kit
ZR small-RNA PAGE Recovery Kit
- Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- Compatible with up to 25% (w/v) polyacrylamide.
The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the "crush and soak" method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.
|Equipment||Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer|
|Purity||RNA is ready for all subsequent analysis and molecular manipulation|
|Sample Source||RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar).|
|Size Range||RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery)|
|Yield||10 µg RNA (binding capacity), ≥6 µl (elution volume)|
Q1: What is the recovery rate?
The recovery rate for fragments 17 to 28 nucleotides is ≥50 %.
Q2: Can the kit be used to recover DNA fragments run on a PAGE gel?
Yes, the kit is compatible with both single- or double-stranded RNA (and DNA) fragments.
Q3: Can the kit be used to purify DNA/RNA fragments > 200 nucleotides run on a PAGE gel?
Yes. Most customer’s do not use PAGE gels for to visualize fragments > 200 nucleotides, but it will be compatible with the kit.
Q4: Is the kit compatible with TBE-Urea PAGE?
Yes, this kit is compatible with TBE-Urea PAGE gels.
Q5: What is the maximum amount of gel that can be processed per prep?
There is no designated upper limit, however it is always more optimal to start with keeping the amount of excised gel as low as possible prior to proceeding with the purification procedure, and scaling up when optimizing gel input amount.
Q6: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
Uncleaved RNA transcripts were extracted from denaturing (8.3M ura) 10% polyacrylamide gel using the ZR small-RNA PAGE Recovery kit. This facilitates the generation of ribozyme-based control devices for gene regulatory activities.Liang JC, et al. A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity. Nucleic Acids Research. 2012.
The ZR Small-RNA PAGE Recovery kit was used to gel purify short RNAs (50-250 bp) from isolated total RNA on a 15% TBE Urea gel for downstream quantitative PCR.Byun, JS et al. ELL facilitates RNA polymerase II pause site entry and release. Nature Communications. 2012.
In vitro transcribed short hairpin RNA constructs were recovered from 20% denaturing polyacrylamide gels using the ZR small-RNA PAGE Recovery kit. This was used to show that influenza A virus panhandle structure could bind to retinoic acid-inducible gene I (RIG-I) and affect type I interferon production.Liu, GQ et al. Influenza A Virus Panhandle Structure Is Directly Involved in RIG-I Activation and Interferon Induction. Journal of Virology. 2015.
|R1003-3-6||RNA Wash Buffer||6 ml||$19.80|
|C1001-50||Collection Tubes||50 Pack||$16.50|
|R1060-2-10||RNA Prep Buffer||10 ml||$19.80|
|R1070-1-10||RNA Recovery Buffer||10 ml||$19.80|
|R1070-2-20||RNA MAX Buffer||20 ml||$59.40|
|W1001-1||DNase/RNase-Free Water||1 ml||$11.00|
|C1007-50||Zymo-Spin IV Columns||50 Pack||$64.90|