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ZR small-RNA PAGE Recovery Kit

R1070


ZR small-RNA PAGE Recovery Kit

R1070

Cat # Name Size Price Quantity
R1070 ZR small-RNA PAGE Recovery Kit 20 Preps $143.00
$107.25
+ -

Documents


RNA (and DNA) from PAGE gels

Highlights


  • Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
  • Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
  • Compatible with up to 25% (w/v) polyacrylamide.

Description


The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the "crush and soak" method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.

Technical Specifications


Equipment Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer
Purity RNA is ready for all subsequent analysis and molecular manipulation
Sample Source RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar).
Size Range RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery)
Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

Product FAQ


Q1: What is the recovery rate?

Q2: Can the kit be used to recover DNA fragments run on a PAGE gel?

Q3: Can the kit be used to purify DNA/RNA fragments > 200 nucleotides run on a PAGE gel?

Q4: Is the kit compatible with TBE-Urea PAGE?

Q5: What is the maximum amount of gel that can be processed per prep?

Q6: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?

Citations


Uncleaved RNA transcripts were extracted from denaturing (8.3M ura) 10% polyacrylamide gel using the ZR small-RNA PAGE Recovery kit. This facilitates the generation of ribozyme-based control devices for gene regulatory activities.

Liang JC, et al. A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity. Nucleic Acids Research. 2012.

The ZR Small-RNA PAGE Recovery kit was used to gel purify short RNAs (50-250 bp) from isolated total RNA on a 15% TBE Urea gel for downstream quantitative PCR.

Byun, JS et al. ELL facilitates RNA polymerase II pause site entry and release. Nature Communications. 2012.

In vitro transcribed short hairpin RNA constructs were recovered from 20% denaturing polyacrylamide gels using the ZR small-RNA PAGE Recovery kit. This was used to show that influenza A virus panhandle structure could bind to retinoic acid-inducible gene I (RIG-I) and affect type I interferon production.

Liu, GQ et al. Influenza A Virus Panhandle Structure Is Directly Involved in RIG-I Activation and Interferon Induction. Journal of Virology. 2015.

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Kit Components