Zymoclean Gel RNA Recovery Kit
- Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- High Recovery: ≥ 80% recovery for RNA > 500 nt.
|Equipment||Microcentrifuge, 37°C-65°C heat source|
|Purity||RNA is ready for all subsequent analysis and molecular manipulation|
|Sample Source||Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.)|
|Size Range||RNA fragments ≥500 nt (≥80% recovery)|
|Yield||10 µg RNA (binding capacity), ≥6 µl (elution volume)|
Q1: Are the columns and wash buffer interchangeable with the RCC-5 Kit?
Q2: Can the Zymoclean be used for samples in solution (without running on a gel)?
Q3: What is the maximum weight of gel slice I can use?
Q4: I ran out of RNA Wash Buffer. Can I use something else?
Q5: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Dimeric RNA was extracted from gel slice using the Zymoclean Gel RNA Recovery kit for Northern analysis of signal recognition particle (SRP) RNA.Rulli Jr SJ, et al. Selective and Nonselective Packaging of Cellular RNAs in Retrovirus Particles. Journal of Virology. 2007.
The Zymoclean Gel RNA Recovery kit was used on agarose gels with RNA derived from Candida albicans strains. Researchers showed new mechanisms that regulates rRNA processing involving 18S, 5.8S and 25S rRNAs.Pendrak, ML et al. Ribosomal RNA processing in Candida albicans. RNA Journal. 2011.
A dsRNA full-length transcript standard was visualized by electrophoresis on a 1% denaturing agarose gel and isolated using the Zymoclean Gel RNA Recovery kit. This rotavirus (RVA) dsRNA was used in qRT-PCR for RVA detection with the limit of detection being 1 genome copy per reaction.Mijatovic-Rustempasic S et al. Sensitive and Specific Quantitative Detection of Rotavirus A by One-Step Real-Time Reverse Transcription-PCR Assay without Antecedent Double-Stranded-RNA Denaturation. Journal of Clinical Microbiology. 2013.