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Highlights
- Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- High Recovery: ≥ 80% recovery for RNA > 500 nt.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
Highlights
- Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- High Recovery: ≥ 80% recovery for RNA > 500 nt.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| W1001-1 | DNase/RNase-Free Water | 1 ml | $11.60 | |
| R1003-3-12 | RNA Wash Buffer | 12 ml | $35.00 | |
| R1011-1-50 | RAD Buffer (RNA Agarose Dissolving Buffer) | 50 ml | $87.60 | |
| R1060-2-25 | RNA Prep Buffer | 25 ml | $46.70 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.50 | |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack | $61.80 | |
Description
Performance
Technical Specifications
| Equipment | Microcentrifuge, 37°C-65°C heat source |
|---|---|
| Purity | RNA is ready for all subsequent analysis and molecular manipulation |
| Sample Source | Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.) |
| Size Range | RNA fragments ≥500 nt (≥80% recovery) |
| Yield | 10 µg RNA (binding capacity), ≥6 µl (elution volume) |
Resources
Documents
FAQ
Yes, the Zymo-Spin IC Columns and RNA Wash Buffer are interchangeable.
No, it’s not optimized for samples in solution. High efficiency recovery is not guaranteed and the success of the purification can be variable.
Up to 400 mg of agarose.
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
Citations
Dimeric RNA was extracted from gel slice using the Zymoclean Gel RNA Recovery kit for Northern analysis of signal recognition particle (SRP) RNA.
Rulli Jr SJ, et al. Selective and Nonselective Packaging of Cellular RNAs in Retrovirus Particles. Journal of Virology. 2007.The Zymoclean Gel RNA Recovery kit was used on agarose gels with RNA derived from Candida albicans strains. Researchers showed new mechanisms that regulates rRNA processing involving 18S, 5.8S and 25S rRNAs.
Pendrak, ML et al. Ribosomal RNA processing in Candida albicans. RNA Journal. 2011.A dsRNA full-length transcript standard was visualized by electrophoresis on a 1% denaturing agarose gel and isolated using the Zymoclean Gel RNA Recovery kit. This rotavirus (RVA) dsRNA was used in qRT-PCR for RVA detection with the limit of detection being 1 genome copy per reaction.
Mijatovic-Rustempasic S et al. Sensitive and Specific Quantitative Detection of Rotavirus A by One-Step Real-Time Reverse Transcription-PCR Assay without Antecedent Double-Stranded-RNA Denaturation. Journal of Clinical Microbiology. 2013.Read More
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