Zymoprep-96 Yeast Plasmid Miniprep

D2005 / D2006 / D2007


Zymoprep-96 Yeast Plasmid Miniprep

D2005 / D2006 / D2007

Cat # Name Size Price Quantity
D2005 Zymoprep-96 Yeast Plasmid Miniprep 2 x 96 Preps $325.00
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D2006 Zymoprep-96 Yeast Plasmid Miniprep 4 x 96 Preps $595.00
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D2007 Zymoprep-96 Yeast Plasmid Miniprep 8 x 96 Preps $998.00
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Documents


Simple solution for yeast plasmid DNA isolation using Zymolyase

Highlights

  • Simple: Quickly and easily rescue plasmid from yeast.
  • Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
  • High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Description

The Zymoprep-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase added in the first solution. There is no need for glass beads or phenol. The Zymoprep-96 incorporates our Zymoprep II kit into a 96-well format resulting in a high-throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.


Applicable For Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
Elution Volume ≥ 10 µl per well
Equipment Centrifuge with microplate carriers
Processing Volume ≤ 1.5 ml of Culture
Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
Size Range Up to 25 kb.
Yield Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures

Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?

Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.

Q2: What is the typical plasmid yield?

Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.

Q3: Can this kit be used to isolate linear plasmid DNA?

Yes

Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?

We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.

Q5: What yeast strains are these kits compatible with?

Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis



Cat # Name Size Price
D2004-1-45 Solution 1 Digestion Buffer 45 ml $88.00
D2004-1-90 Solution 1 Digestion Buffer 90 ml $146.00
D2004-2-45 Solution 2 Lysis Buffer 45 ml $88.00
D2004-2-90 Solution 2 Lysis Buffer 90 ml $146.00
D2004-3-180 Solution 3 Neutralizing Buffer 180 ml $146.00
D2004-3-90 Solution 3 Neutralizing Buffer 90 ml $88.00
D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml $33.00
D4003-2-48 DNA Wash Buffer (Concentrate) 48 ml $60.00
C1001-50 Collection Tubes 50 Pack $15.00
C2002 Collection Plate 2 Plates $22.00
C2003 Elution Plate 2 Plates $19.00
C2004 Zymo-Spin I-96 Plate 2 Plates $142.00
P1001-2 96-Well Block 2 Blocks $18.00
C2007-8 96-Well Plate Cover Foil 8 Foils $18.00
C2011-2 Air Permeable Sealing Cover 2 Pack $12.00
C2011-4 Air Permeable Sealing Cover 4 Pack $24.00
C2011-8 Air Permeable Sealing Cover 8 Pack $42.00