Zymoprep Yeast Plasmid Miniprep II

D2004


Zymoprep Yeast Plasmid Miniprep II

Cat # Name Size Price Quantity
D2004 Zymoprep Yeast Plasmid Miniprep II 50 Preps $138.00
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Zymoprep™ Yeast Plasmid Miniprep II

Highlights

  • Simple: Quickly and easily rescue plasmid from yeast.
  • Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
  • High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Description

The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.


Applicable For Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
Elution Volume ≥ 10 µl
Equipment Microcentrifuge & Heat Block/Bath
Processing Volume ≤ 1.5 ml of Culture
Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
Size Range Up to 23 kb.
Yield Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures

Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?

Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.

Q2: What is the typical plasmid yield?

Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.

Q3: Can this kit be used to isolate linear plasmid DNA?

Yes

Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?

We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.

Q5: What yeast strains are these kits compatible with?

Any strains susceptible to Zymolyase, which includes: Susceptible fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis



Cat # Name Size Price
E1004 Zymolyase 1000 U $75.00
D2004-1-10 Solution 1 Digestion Buffer 10 ml $18.00
D2004-2-10 Solution 2 Lysis Buffer 10 ml $18.00
D2004-3-20 Solution 3 Neutralizing Buffer 20 ml $18.00
D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml $10.00
C1001-50 Collection Tubes 50 Pack $15.00
C1003-50 Zymo-Spin I Columns 50 Pack $50.00