Zymoprep Yeast Plasmid Miniprep II
- Simple: Quickly and easily rescue plasmid from yeast.
- Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
- High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.
|Applicable For||Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications|
|Elution Volume||≥ 10 µl|
|Equipment||Microcentrifuge & Heat Block/Bath|
|Processing Volume||≤ 1.5 ml of Culture|
|Sample Source||Cell Culture (Colonies/Patches or Liquid Culture)|
|Size Range||Up to 23 kb.|
|Yield||Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures|
Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?
Q2: What is the typical plasmid yield?
Q3: Can this kit be used to isolate linear plasmid DNA?
Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?
Q5: What yeast strains are these kits compatible with?
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