ZymoPURE II Plasmid Gigaprep Kit
- Fast: 50 minute Gigapreps
- Highest Yield: Purify up to 10 mg of plasmid DNA directly from a spin-column.
- Ultra-Pure: EndoZero, vaccine grade, and transfection ready.
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 10 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Gigaprep kit can process up to 2.5 L of culture in less than 50 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with a vacuum instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl) , EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Gigaprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
|Applicable For||Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.|
|Binding Capacity||10 mg|
|Culture Input||≤ 2.5 L|
|Elution Volume||≥ 2 ml|
|Endotoxin Levels||≤ 0.025 EU/µg Plasmid DNA|
|Equipment||Vacuum/vacuum manifold and swinging bucket centrifuge|
|Processing Time||50 min|
|Purity||Typical Abs 260/280 ≥1.8 and Abs 260/230 ≥ 2.0|
|Recommended Sample Volume||≤ 2.5 L|
|Size Range||Up to 200 kb|
|Yield||≤ 10 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)|
Q1: Can I process more than the recommended volume of bacterial culture or use enriched growth media?
Q2: Is there a protocol for low-copy number plasmid DNA?
Q3: Can the ZymoPURE™ kits be used to isolate large plasmid constructs (BAC/PAC)?
Q4: Can an in-house vacuum line be used with the EZ Vac Vacuum Manifold?
Q5: Are the ZymoPURE™ kits compatible with any commercially available vacuum manifold?
Q6: What type of vacuum pump do you recommend?
Q7: Can the ZymoPURE™ Kits be used with other bacteria?
Q8: I ran out of ZymoPURE™ Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Q9: What is the composition of the ZymoPURE™ Elution buffer?
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