ZymoPURE Plasmid Miniprep Kit Sample


ZymoPURE Plasmid Miniprep Kit Sample

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ZymoPURE Plasmid Miniprep Kit Sample

Highlights

  • Highest Yield: Purify up to 100 µg of plasmid DNA in as little as 25 µl directly from a spin-column.
  • Transfection-Grade: 50,000 times fewer endotoxins than industry leading minipreps.
  • BAC/YAC/PAC Ready: Purify DNA up to ~200 kb.
Description

Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

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Applicable For Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.
Binding Capacity 100 µg
Culture Input ≤ 5 mL
Elution Volume 25 µl
Endotoxin Levels ≤ 1 EU/µg
Equipment Microcentrifuge and/or vacuum manifold
Format Spin Column
Processing Time 15 min
Purity Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Recommended Sample Volume ≤ 5.0 ml
Size Range Up to ~200 kb
Supplemental Info
Yield Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.

10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

No, the ZymoPURE™ kits are only compatible with ZymoPURE™ Wash 2 and we cannot disclose a substitution due to the sensitive nature of the recipe. Additional ZymoPURE™ Wash 2 can be purchased separately.

The vacuum pump should be a single or double-staged unit capable of producing at least 400 mm Hg pressure at the vacuum manifold. If less pressure is applied, centrifuge the column prior to washing to remove any residual lysate/buffer remaining in the matrix.

Yes, any vacuum that uses standard luer-lock connectors is compatible.

Yes, however, the pressure needs to be around 400 mm Hg. Users should take caution, as the pressure of an in-house vacuum line can fluctuate drastically or be significantly reduced, depending on the demand in the building.

Yes, please contact technical support for an application note.

Yes, the low-copy protocol can be found in the Appendix of the kit protocol.

Yes, however, more attention is required during the lysis and steps. We recommend centrifuging/pelleting the neutralized lysate before loading onto the supplied syringe/bottle-top filter. For high copy plasmids using more culture can cause overloading and subsequent clogging of columns, which can result in poor DNA yield and/or quality.

Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. To increase elution of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ºC) and increasing the incubation time on column up to 10 minutes prior to centrifugation.

Yes, the standard protocol should work with other gram-negative bacteria. However, the user will need to validate the protocol for their particular species. For gram-positive bacteria, please refer to the Gram-Positive Bacteria Protocol in the Appendix of the kit protocol.




Cat # Name Size Price
C1086-50 Zymo-Spin II-PX Columns 50 Pack $72.17
C1001-50 Collection Tubes 50 Pack $16.50
D4200-6-23 ZymoPURE Wash 2 (Concentrate) 23 ml $44.00
D4200-4-3 ZymoPURE Binding Buffer 3 ml $11.00
D4200-5-20 ZymoPURE Wash 1 20 ml $18.70
D4200-5-320 ZymoPURE Wash 1 320 ml $214.50
D4200-7-6 ZymoPURE Elution Buffer 6 ml $12.10
D4200-6-28 ZymoPURE Wash 2 (Concentrate) 28 ml $47.30
D4200-7-12 ZymoPURE Elution Buffer 12 ml $22.00
D4200-3-210 ZymoPURE P3 (Yellow) 210 ml $55.00
D4200-3-3 ZymoPURE P3 (Yellow) 3 ml $11.00
D4200-4-110 ZymoPURE Binding Buffer 110 ml $42.90
D4200-4-14 ZymoPURE Binding Buffer 14 ml $13.20
D4200-2-210 ZymoPURE P2 (Green) 210 ml $55.00
D4200-2-3 ZymoPURE P2 (Green) 3 ml $11.00
D4200-3-100 ZymoPURE P3 (Yellow) 100 ml $31.90
D4200-3-13 ZymoPURE P3 (Yellow) 13 ml $13.20
D4200-1-210 ZymoPURE P1 (Red) 210 ml $68.20
D4200-1-3 ZymoPURE P1 (Red) 3 ml $11.00
D4200-2-100 ZymoPURE P2 (Green) 100 ml $31.90
D4200-2-13 ZymoPURE P2 (Green) 13 ml $13.20
D4200-1-100 ZymoPURE P1 (Red) 100 ml $39.60
D4200-1-13 ZymoPURE P1 (Red) 13 ml $13.20
D4200-7-30 ZymoPURE Elution Buffer 30 ml $41.80