Zyppy-96 Plasmid Kit
Zyppy-96 Plasmid Kit
- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
- Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
|Applicable For||Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.|
|Elution Volume||≥ 30 µl|
|Equipment||Centrifuge with micro plate carriers|
|Processing Time||45 min|
|Purity||Typical Abs 260/280 ≥ 1.8.|
|Size Range||Up to 25 kb|
|Yield||Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.|
Q1: Can E.coli grown in enriched growth media be used with this kit?
Q2: What is the composition of the Zyppy Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q3: Can the purified plasmid be transfected into eukaryotic cell lines?
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?
No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.
Q5: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?
≤ 50 Endotoxin Units/µg of plasmid DNA.
Q6: I accidentally left my Neutralization Buffer at room temperature. Will it be okay?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4°C as soon as possible.
Q7: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.
|D4036-5-60||Zyppy Elution Buffer||60 ml||$72.00|
|D4041-1-30||Deep Blue Lysis Buffer||30 ml||$46.00|
|D4041-1-48||Deep Blue Lysis Buffer||48 ml||$69.00|
|D4041-4-100||Neutralization/Clearing Buffer||100 ml||$80.00|
|D4041-4-200||Neutralization/Clearing Buffer||200 ml||$137.00|
|D4036-5-30||Zyppy Elution Buffer||30 ml||$38.00|
|D4036-5-100||Zyppy Elution Buffer||100 ml||$90.00|
|D4036-4-48||Zyppy Wash Buffer (Concentrate)||48 ml||$49.00|
|D4036-4-24||Zyppy Wash Buffer (Concentrate)||24 ml||$34.00|
|D4036-3-160||Endo-Wash Buffer||160 ml||$56.00|
|D4036-3-120||Endo-Wash Buffer||120 ml||$49.00|
|C2007-24||96-Well Plate Cover Foil||24 Foils||$43.00|
|C2007-6||96-Well Plate Cover Foil||6 Foils||$12.00|
|C2011-8||Air Permeable Sealing Cover||8 Pack||$42.00|
|C2011-4||Air Permeable Sealing Cover||4 Pack||$24.00|
|C2011-2||Air Permeable Sealing Cover||2 Pack||$12.00|
|C2007-12||96-Well Plate Cover Foil||12 Foils||$22.00|
|C2004||Zymo-Spin I-96 Plate||2 Plates||$142.00|
|P1001-2||96-Well Block||2 Blocks||$18.00|
|C2002||Collection Plate||2 Plates||$22.00|
|C2003||Elution Plate||2 Plates||$19.00|