Zyppy-96 Plasmid Kit

D4041 / D4042 / D4043


Zyppy-96 Plasmid Kit

D4041 / D4042 / D4043

Cat # Name Size Price Quantity
D4041 Zyppy-96 Plasmid Kit 2 x 96 Preps $370.00
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D4042 Zyppy-96 Plasmid Kit 4 x 96 Preps $662.00
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D4043 Zyppy-96 Plasmid Kit 8 x 96 Preps $1,190.00
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Pellet-free, high-quality plasmid DNA in only 8 minutes

Highlights

  • Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
  • High Quality: Ready for PCR, sequencing, cloning, and transfection.
  • Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
Description

The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.


Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume ≥ 30 µl
Equipment Centrifuge with micro plate carriers
Processing Time 45 min
Purity Typical Abs 260/280 ≥ 1.8.
Size Range Up to 25 kb
Yield Up to 10 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.

Q1: Can E.coli grown in enriched growth media be used with this kit?

No

Q2: What is the composition of the Zyppy Elution buffer?

10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

Q3: Can the purified plasmid be transfected into eukaryotic cell lines?

Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.

Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?

No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.

Q5: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?

≤ 50 Endotoxin Units/µg of plasmid DNA.

Q6: I accidentally left my Neutralization Buffer at room temperature. Will it be okay?

Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4°C as soon as possible.

Q7: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?

Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.



Cat # Name Size Price
D4036-3-120 Endo-Wash Buffer 120 ml $49.00
D4036-3-160 Endo-Wash Buffer 160 ml $56.00
D4036-4-24 Zyppy Wash Buffer (Concentrate) 24 ml $34.00
D4036-4-48 Zyppy Wash Buffer (Concentrate) 48 ml $49.00
D4036-5-100 Zyppy Elution Buffer 100 ml $90.00
D4036-5-30 Zyppy Elution Buffer 30 ml $38.00
D4036-5-60 Zyppy Elution Buffer 60 ml $72.00
D4041-1-30 Deep Blue Lysis Buffer 30 ml $46.00
D4041-1-48 Deep Blue Lysis Buffer 48 ml $69.00
D4041-4-100 Neutralization/Clearing Buffer 100 ml $80.00
D4041-4-200 Neutralization/Clearing Buffer 200 ml $137.00
C2002 Collection Plate 2 Plates $22.00
C2003 Elution Plate 2 Plates $19.00
C2004 Zymo-Spin I-96 Plate 2 Plates $142.00
P1001-2 96-Well Block 2 Blocks $18.00
C2007-12 96-Well Plate Cover Foil 12 Foils $22.00
C2007-24 96-Well Plate Cover Foil 24 Foils $43.00
C2007-6 96-Well Plate Cover Foil 6 Foils $12.00
C2011-2 Air Permeable Sealing Cover 2 Pack $12.00
C2011-4 Air Permeable Sealing Cover 4 Pack $24.00
C2011-8 Air Permeable Sealing Cover 8 Pack $42.00