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    Zyppy-96 Plasmid Kit

    High-throughput purification of ultra-pure plasmid DNA from 750 µl of overnigh E. coli culture without pelleting cells.

    Highlights

    • Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
    • High Quality: Ready for PCR, sequencing, cloning, and transfection.
    • Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Highlights

    • Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
    • High Quality: Ready for PCR, sequencing, cloning, and transfection.
    • Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Cat # Name Size Price Quantity
    D4041 Zyppy-96 Plasmid Kit 2 x 96 Preps $457.70
    - +
    D4042 Zyppy-96 Plasmid Kit 4 x 96 Preps $818.00
    - +
    D4043 Zyppy-96 Plasmid Kit 8 x 96 Preps $1,470.60
    - +
    Cat # Name Size Price
    D4036-5-60 Zyppy Elution Buffer 60 ml $84.00
    D4041-1-30 Deep Blue Lysis Buffer 30 ml $53.70
    D4041-1-48 Deep Blue Lysis Buffer 48 ml $80.50
    D4041-4-100 Neutralization/Clearing Buffer 100 ml $93.30
    D4041-4-200 Neutralization/Clearing Buffer 200 ml $159.90
    D4036-5-30 Zyppy Elution Buffer 30 ml $44.40
    D4036-5-100 Zyppy Elution Buffer 100 ml $105.10
    D4036-4-48 Zyppy Wash Buffer (Concentrate) 48 ml $57.20
    D4036-4-24 Zyppy Wash Buffer (Concentrate) 24 ml $39.70
    D4036-3-160 Endo-Wash Buffer 160 ml $65.30
    D4036-3-120 Endo-Wash Buffer 120 ml $57.20
    C2007-24 96-Well Plate Cover Foil 24 Foils $44.30
    C2007-6 96-Well Plate Cover Foil 6 Foils $12.40
    C2011-8 Air Permeable Sealing Cover 8 Pack $43.30
    C2011-4 Air Permeable Sealing Cover 4 Pack $24.70
    C2011-2 Air Permeable Sealing Cover 2 Pack $12.40
    C2007-12 96-Well Plate Cover Foil 12 Foils $22.70
    C2004 Zymo-Spin I-96 Plate 2 Plates $165.70
    P1001-2 96-Well Block 2 Blocks $21.00
    C2002 Collection Plate 2 Plates $25.60
    C2003 Elution Plate 2 Plates $22.10

    Description

    The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.

    Performance

    Technical Specifications
    Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
    Elution Volume ≥ 30 µl
    Equipment Centrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches)
    Processing Time 45 min
    Purity Typical Abs 260/280 ≥ 1.8.
    Size Range Up to 25 kb
    Yield Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.

    Resources

    Documents

    FAQ

    No

    10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

    Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.

    No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.

    ≤ 50 Endotoxin Units/µg of plasmid DNA.

    Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4°C as soon as possible.

    Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.

    Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.

    No, the sediment inside the buffer is insoluble, but it needs to be completely resuspended in the buffer prior to use for optimal performance.

    Citations

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