Zyppy-96 Plasmid MagBead Miniprep

D4100 / D4101 / D4102


Zyppy-96 Plasmid MagBead Miniprep

D4100 / D4101 / D4102

Cat # Name Size Price Quantity
D4100 Zyppy-96 Plasmid MagBead Miniprep 2 x 96 Preps. $315.00
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D4101 Zyppy-96 Plasmid MagBead Miniprep 4 x 96 Preps. $555.00
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D4102 Zyppy-96 Plasmid MagBead Miniprep 8 x 96 Preps. $997.00
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Pellet-free, high-quality plasmid DNA in only 8 minutes

Highlights

  • Pellet-Free: Rapid direct lysis procedure omits cell-pelleting and resuspension steps
  • High Quality: Ready for PCR, sequencing, cloning, and transfection
  • Ideal for Synthetic Biology: Fastest, high-throughput automated method for preparing high-quality plasmid DNA.
Description

The Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization Buffer. Plasmid DNA is then purified using the featured magnetic bead based technology. This straightforward procedure is compatible for either automated or manual processing. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.


Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume ≥ 30 µl
Equipment Magnetic Stand, Heating Element, or Liquid Handler
Processing Time 60 min
Purity Typical Abs 260/280 ≥1.8.
Size Range Up to 25 kb
Yield Up to 5 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli processed.

Q1: What is the composition of the Zyppy Elution buffer?

10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

Q2: Can the purified plasmid be transfected into eukaryotic cell lines?

Yes, all our plasmid preps have an endo-wash step to remove endotoxin very effectively and produce transfection ready high-quality plasmid DNA.

Q3: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?

No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.

Q4: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?

≤ 50 Endotoxin Units/µg of plasmid DNA.

Q5: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?

Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.


“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min.  Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”

- Rebecca N.

“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard).  The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”

- Richard F.

"The results gave what they promised!  Clean DNA.">/em<

- Pamela R (UT Medicine San Antonio)

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Cat # Name Size Price
D4036-2-100 Neutralization Buffer (Yellow) 100 ml $81.00
D4036-2-200 Neutralization Buffer (Yellow) 200 ml $137.00
D4036-3-120 Endo-Wash Buffer 120 ml $49.00
D4036-3-240 Endo-Wash Buffer 240 ml $85.00
D4036-3-60 Endo-Wash Buffer 60 ml $34.00
D4036-4-48 Zyppy Wash Buffer (Concentrate) 48 ml $49.00
D4036-5-100 Zyppy Elution Buffer 100 ml $90.00
D4036-5-30 Zyppy Elution Buffer 30 ml $38.00
D4036-5-60 Zyppy Elution Buffer 60 ml $72.00
D4041-1-30 Deep Blue Lysis Buffer 30 ml $46.00
D4041-1-48 Deep Blue Lysis Buffer 48 ml $69.00
D4100-1-10 MagClearing Beads 10 ml $69.00
D4100-1-20 MagClearing Beads 20 ml $125.00
D4100-1-40 MagClearing Beads 40 ml $224.00
D4100-2-16 MagBinding Beads 16 ml $166.00
D4100-2-8 MagBinding Beads 8 ml $92.00
C2002 Collection Plate 2 Plates $22.00
C2003 Elution Plate 2 Plates $19.00
P1001-2 96-Well Block 2 Blocks $18.00
C2007-12 96-Well Plate Cover Foil 12 Foils $22.00
C2007-24 96-Well Plate Cover Foil 24 Foils $43.00
C2007-6 96-Well Plate Cover Foil 6 Foils $12.00
C2011-2 Air Permeable Sealing Cover 2 Pack $12.00
C2011-4 Air Permeable Sealing Cover 4 Pack $24.00
C2011-8 Air Permeable Sealing Cover 8 Pack $42.00