- Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
|Applicable For||Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.|
|Elution Volume||≥ 30 µl|
|Equipment||Microcentrifuge and/or a vacuum manifold.|
|Processing Time||8 min|
|Purity||Typical Abs260/280 ≥1.8.|
|Size Range||Up to 25 kb|
|Yield||Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.|
Q1: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.
Q2: I accidently left my Neutralization Buffer at room temperature. Will it be okay?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible.
Q3: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?
≤ 50 Endotoxin Units/µg of plasmid DNA.
Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?
No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.
Q5: Can the purified plasmid be transfected into eukaryotic cell lines?
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
Q6: What is the composition of the Zyppy Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q7: Can E.coli grown in enriched growth media be used with this kit?
Yes, pellet and resuspend E.coli in water or TE before proceeding with the protocol.
“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min. Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”
- Rebecca N.
“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard). The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”
- Richard F.
"The results gave what they promised! Clean DNA."
- Pamela R (UT Medicine San Antonio)
|D4036-1-12||7X Lysis Buffer (Blue)||12 ml||$35.00|
|D4036-1-48||7X Lysis Buffer (Blue)||48 ml||$69.00|
|D4036-1-6||7X Lysis Buffer (Blue)||6 ml||$15.00|
|D4036-2-160||Neutralization Buffer (Yellow)||160 ml||$127.00|
|D4036-2-20||Neutralization Buffer (Yellow)||20 ml||$12.00|
|D4036-2-40||Neutralization Buffer (Yellow)||40 ml||$35.00|
|D4036-3-120||Endo-Wash Buffer||120 ml||$49.00|
|D4036-3-15||Endo-Wash Buffer||15 ml||$10.00|
|D4036-3-30||Endo-Wash Buffer||30 ml||$18.00|
|D4036-4-12||Zyppy Wash Buffer (Concentrate)||12 ml||$18.00|
|D4036-4-48||Zyppy Wash Buffer (Concentrate)||48 ml||$49.00|
|D4036-4-6||Zyppy Wash Buffer (Concentrate)||6 ml||$10.00|
|D4036-5-20||Zyppy Elution Buffer||20 ml||$25.00|
|D4036-5-5||Zyppy Elution Buffer||5 ml||$9.00|
|C1001-50||Collection Tubes||50 Pack||$15.00|
|C1019-50||Zymo-Spin IIN Columns||50 Pack||$54.00|