- Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
|Applicable For||Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.|
|Elution Volume||≥ 30 µl|
|Equipment||Microcentrifuge and/or a vacuum manifold.|
|Processing Time||8 min|
|Purity||Typical Abs260/280 ≥1.8.|
|Size Range||Up to 25 kb|
|Yield||Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.|
Q1: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Q2: I accidently left my Neutralization Buffer at room temperature. Will it be okay?
Q3: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?
Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?
Q5: Can the purified plasmid be transfected into eukaryotic cell lines?
Q6: What is the composition of the Zyppy Elution buffer?
Q7: Can E.coli grown in enriched growth media be used with this kit?
The Zyppy Plasmid MiniPrep was used to isolate plasmids transformed with the Mix & Go Competent Cells containing 16s rRNA eubacteria and actinobacteria genes. These clone libraries were used to identify the microbial community and characterize functional genes responsible for biotransformation in composted food waste.
The ZR Soil Microbe DNA MiniPrep was used to isolate microbial DNA from composted food waste and the resulting clone libraries containing 16s rRNA eubacteria and actinobacteria genes were used to identify the microbial community and characterize functional genes responsible for biotransformation.
The Zyppy Plasmid MiniPrep was used to isolate vectors in the construction of synthetic yeast promoters. Researchers showed that decreasing nucleosome affinity corresponds with increasing promoter strength and expression levels higher than native species.
Plasmids were transformed with high efficiency using the Frozen-EZ Yeast Transformation II Kit in the construction of synthetic yeast promoters. Researchers showed that decreasing nucleosome affinity correlated with increasing promoter strength and expression levels higher than native species.
The Zyppy™ Plasmid MiniPrep was utilized to isolate high quality plasmids for phylogenetic analysis to study methanogen diversity in animal hosts. The data was used to demonstrate the potential to develop markers to track sources of fecal pollution.Ufnar, JA. Et al. (2007). Development of a swine-specific fecal pollution marker based on host differences in methanogen mcrA genes. Appl. Environ. Microbiolo., 73(16), 5209-5217.
“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min. Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”
- Rebecca N.
“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard). The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”
- Richard F.
"The results gave what they promised! Clean DNA."
- Pamela R (UT Medicine San Antonio)