ZymoPURE Plasmid Kits

ZymoPURE Plasmid kits are the fastest and simplest plasmid kits available to efficiently isolate transfection-grade plasmid DNA from E. coli (up to 3 mg in 16 minutes). These plasmid kits feature our advanced ZymoPURE purification technology, including a modified alkaline lysis system and patented binding chemistry, enabling the recovery of highly concentrated plasmid DNA (up to 6 µg/µl) directly from a spin column using microcentrifuge.

Instead of time-consuming gravity flow columns found in conventional transfection-grade plasmid kits, these plasmid kits utilize vacuum filtration and binding for rapid sample processing. Furthermore, the eluted plasmid DNA is ready for immediate use, without the need for alcohol precipitation. Resulting in the purification of transfection-grade plasmid DNA up to 9 times faster then traditional methods.

As an added convenience, these plasmid kits contain colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The wash regimen of these plasmid kits has been optimized to ensure the eluted DNA is free of endotoxins, salt, protein, and RNA resulting in plasmids that are suitable for sensitive applications including transfection, in vivo injections, gene editing, recombinant viral vector production, PCR and sequencing. 

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Comparison Table

Catalog # Product Binding Capacity Elution Volume Culture Input Endotoxin Levels Format
D4208T ZymoPURE Plasmid Miniprep 100 µg ≥ 25 µl ≤ 5 mL Culture ≤ 1 EU/µg DNA Spin-Column
D4200 ZymoPURE II Plasmid Midiprep 1.2 mg ≥ 150 µl ≤ 50 ml ≤ 0.025 EU/µg DNA Spin-Column
D4202 ZymoPURE II Plasmid Maxiprep 3.0 mg ≥ 300 µl ≤ 150 ml ≤ 0.025 EU/µg DNA Spin-Column
D4204 ZymoPURE II Plasmid Gigaprep 25mg ≥ 3 ml ≤ 2.5 L ≤ 0.025 EU/µg DNA Spin-Column
D4213 ZymoPURE-Express Plasmid Midiprep 1.2 mg ≥ 150 µl 25 mL ≤ 1 EU/µg Plasmid DNA Spin-Column