- Fastest: Simple 20 minute Midipreps.
- Highest Yield: Purify up to 1.2mg of plasmid DNA directly from a spin-column.
- Ultra-Pure: EndoZero, vaccine grade, and transfection ready.
|Applicable For||Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.|
|Binding Capacity||1.2 mg|
|Culture Input||≤ 150 ml|
|Elution Volume||≥ 200 µl|
|Endotoxin Levels||≤ 0.025 EU/µg Plasmid DNA|
|Equipment||Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.|
|Processing Time||20 min|
|Purity||Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0|
|Recommended Sample Volume||≤ 150 ml|
|Size Range||Up to 200 kb|
|Yield||≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)|
Q1: What is the composition of the ZymoPURE Elution buffer?
Q2: Can the ZymoPURE Kits be used with other bacteria?
Q3: What type of vacuum pump do you recommend?
Q4: Are the ZymoPURE kits compatible with any commercially available vacuum manifold?
Q5: Can an in-house vacuum line be used with the EZ Vac Vacuum Manifold?
Q6: I ran out of ZymoPURE Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Q7: Is there a protocol for low-copy number plasmid DNA?
Q8: Can I process more than the recommended volume of bacterial culture or use enriched growth media?
Q9: Can the ZymoPURE kits be used to isolate large plasmid constructs (BAC/PAC)?
“Perfect! Finally, we got 600ug plasmid from 150ml E.coli culture in half an hour.”
- B. P., Los Angeles Biomedical Research Institute at Harbor-UCLA
“We were impressed by the high yield of plasmid DNA from this kit! From a 150 mL culture, I was able to isolate 0.912 mg DNA, in the same amount of time (and effort) I could get about half as much DNA from the comparable Qiagen kit.”
- C. S., Abbott Laboratories
“I was very satisfied with this product. It produced high DNA concentration eluting in 400 µl using the above mentioned cell line, which would generally only be obtainable with other kits eluting in much smaller volumes. This product is also miles ahead of other general Maxiprep kits in terms of time required for plasmid extraction.”
- J. P., University of Pretoria