- Highest Yield: Purify up to 100 µg of plasmid DNA in as little as 25 µl directly from a spin-column.
- Transfection-Grade: 50,000 times fewer endotoxins than industry leading minipreps.
- BAC/YAC/PAC Ready: Purify DNA up to ~200 kb.
Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
|Applicable For||Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.|
|Binding Capacity||100 µg|
|Culture Input||≤ 5 mL|
|Elution Volume||25 µl|
|Endotoxin Levels||≤ 1 EU/µg|
|Equipment||Microcentrifuge and/or vacuum manifold|
|Processing Time||15 min|
|Purity||Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0|
|Recommended Sample Volume||≤ 5.0 ml|
|Size Range||Up to 25 kb|
|Yield||Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.|
Q1: What is the composition of the ZymoPURE™ Elution buffer?
Q2: I ran out of ZymoPURE™ Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Q3: What type of vacuum pump do you recommend?
Q4: Are the ZymoPURE kits compatible with any commercially available vacuum manifold?
Q5: Can an in-house vacuum line be used with the EZ Vac Vacuum Manifold?
Q6: Can the ZymoPURE Kits be used with other bacteria?
Q7: Is there a protocol for low-copy number plasmid DNA?
Q8: Can I process more than the recommended volume of bacterial culture or use enriched growth media?
Q9: Can the ZymoPURE kits be used to isolate large plasmid constructs (BAC/PAC)?
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