- Fastest: Simple 20 minute Midipreps.
- Highest Yield: Purify up to 400 µg of plasmid DNA directly from a spin-column.
- Ultra-Pure: EndoZero, vaccine grade, and transfection ready.
|Applicable For||Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.|
|Binding Capacity||400 µg|
|Culture Input||≤ 50 ml|
|Elution Volume||≥ 100 µl|
|Endotoxin Levels||≤ 0.025 EU/µg Plasmid DNA|
|Equipment||Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge|
|Processing Time||20 min|
|Purity||Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0|
|Size Range||Up to 200 kb|
|Yield||≤ 400 µg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)|
Q1: What is the composition of the ZymoPURE Elution buffer?
Q2: Can the ZymoPURE Kits be used with other bacteria?
Q3: What type of vacuum pump do you recommend?
Q4: Are the ZymoPURE kits compatible with any commercially available vacuum manifold?
Q5: Can an in-house vacuum line be used with the EZ Vac Vacuum Manifold?
Q6: I ran out of ZymoPURE Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Q7: Is there a protocol for low-copy number plasmid DNA?
Q8: For low-copy plasmids, can I exceed the recommended volume of bacterial culture or use enriched growth media?
Q9: Can the ZymoPURE kits be used to isolate large plasmid constructs (BAC/PAC)?
“It went beyond my expectation. The protocol is very easy to follow. Very good DNA yields and the DNA was high quality”
- J. G., Yale University
“This kit it pretty great, midiprep DNA in about the time it takes to do a miniprep.”
- T. W., Northeastern University
“My student had an easy time with this kit also, and he had never done a DNA prep before.”
- K. G., Friedrich-Alexander University Erlangen-Nuremberg