The His-Spin Protein Miniprep™ provides a fast method for His-tagged protein purification. The easy-to-follow procedure is based on a nickel-charged His-Affinity Gel (IMAC) protein purification, and the unique Zymo-Spin™ Technology. Up to 1 mg of His-tagged protein can be eluted into ≥ 100 μl of the provided His-Elution Buffer in only 5 minutes. The purified protein is ideal for enzymatic assays, protein biochemical analyses, SDS PAGE, as well as other protein based applications. The straightforward spin-wash-elute protocol dramatically simplifies protein purification: get results in minutes, not hours!

• Purification Time – 5 minutes
• Capacity – up to 1 mg
• Protein Purity – Electrophoretically pure
• Elution Volume – 100-200 μl (optimal 150 μl)
• Elution Method – Imidazol gradient
• Affinity Matrix – Nickel charged agarose

The Strep-Spin Protein Miniprep Kit™ provides a fast purification technology for Strep-tagged proteins. The procedure is based on a novel Strep-Tactin® XT Superflow® resin which binds efficiently to Twin-Strep-tag® as well as single Strep-tag. Up to 450 μg of Strep-tagged protein can be eluted in only 7 minutes. The purified protein is ideal for enzymatic assays, protein biochemical analyses, SDS-PAGE and other applications. The straightforward spin-wash-elute protocol dramatically simplifies protein purification: get results in minutes, not hours!

• Purification Time – 7 minutes
• Capacity – up to 450 μg
• Protein Purity – Electrophoretically pure
• Elution Volume – Recommended 3 x 200 μl
• Elution Method – Biotin excess
• Affinity Matrix – Strep-Tactin® XT Superflow® 50%

The MBP-Spin Protein Miniprep Kit™ provides a fast purification technology for MBP-tagged proteins. The easy-to-follow procedure is based on an affinity matrix composed of amylose resin to specifically bind proteins fused to maltose-binding protein (MBP), and the unique Zymo-Spin™ Technology. Up to 1 mg of MBP-tagged protein can be eluted into ≥ 200 μl of the provided MBP-Elution Buffer in only 6 minutes. The purified protein is ultra-pure and is ideal for enzymatic assays, biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin-wash-elute protocol dramatically simplifies protein purification: get results in minutes, not hours!

• Purification Time – 6 minutes
• Capacity – up to 1 mg
• Protein Purity – Electrophoretically pure
• Elution Volume – ≥ 200 μl
• Elution Method – Maltose excess
• Affinity Matrix – Amylose Resin