His-Spin Protein Miniprep
P2001 / P2002
- Fast: Purify His-tagged proteins from cell lysates in less than 5 minutes.
- Simple: Prepare pure protein for small-scale studies using a spin-column.
- Convenient: No special instrumentation needed other than a benchtop microcentrifuge.
The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
|Affinity Matrix||Nickel charged agarose|
|Binding Capacity||1 mg|
|Elution Method||Imidazole gradient|
|Elution Volume||100 - 200 µl (150 µl is optimal)|
|Principle of Technology||Based on a nickel-charged His-Affinity Gel (IMAC).|
|Processing Time||5 minutes|
|Product Storage||Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.|
|Protein Purity||Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.|
|Sample Type||Cell lysates or other complex protein mixtures containing His-tagged proteins.|
Q1: Is the product suitable for eukaryotic expression system (HEK cells)?
Q2: Can less than 100 µl of His-Elution Buffer be used for the elution step?
Q3: What might reduce protein yields?
Q4: Which chemicals/reagents are not compatible with the kit?
Q5: How do you purify insoluble proteins?
Q6: Can the kit be used to purify membrane proteins?
Q7: Which pH should be used with the kit?
Q8: How do you improve protein quality/purity?
Q9: Is there a recommended lysis protocol?
Q10: Which chemicals/reagents are compatible with the kit?
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