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    DNA/RNA Shield


    Cat # Name Size Price Quantity
    R1100-50 DNA/RNA Shield 50 ml $70.00
    - +
    R1100-250 DNA/RNA Shield 250 ml $248.20
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    R1200-25 DNA/RNA Shield (2X Concentrate) 25 ml $70.00
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    R1200-125 DNA/RNA Shield (2X Concentrate) 125 ml $248.20
    - +

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    Highlights

    • Protect DNA and RNA in any biological sample without cold chain
    • Pathogen inactivation (bacteria, virus, eukaryotic)
    • Streamlined purification – universally compatible and automatable
    Description & Documents Specifications FAQ Citations

    Documents


    Product Description


    DNA/RNA Shield reagent is a DNA and RNA stabilization solution for nucleic acids in any biological sample. This DNA and RNA stabilization solution preserves the genetic integrity and expression profiles of samples at ambient temperatures and completely inactivates infectious agents (viruses, bacteria, fungi, & parasites). The unique DNA and RNA stabilization solution also prevents degradation from freeze-thaw cycling and unexpected freezer failures.

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    Documents

    Technical Specifications

    Applicable For Next-Generation Sequencing, qPCR, microarray.
    Device Specs Reagent
    Reagent Storage Ambient temperature
    Sample Collection 10% (v/v or w/v)
    Sample Source Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.
    Stability RNA: Ambient temperature
    (4°C- 30°C) at least 1 month
    DNA: Ambient temperature
    (4°C- 30°C) at least 2 years
    DNA & RNA: Frozen
    (< -20°C): Indefinitely

    Resources


    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

    No, tissues do not need to be homogenized.

    Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.

    Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 ul of FACS sorted cells into minimally 400 ul of DNA/RNA Shield).

    DNA & RNA (<-20°C): Indefinitely

    RNA > 1 month DNA > 2 years

    DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).

    Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).

    Avoid mixing the reagent and bleach (sodium hypochlorite) or other strong oxidizing agents and acids. Clean up any accidental spills with water first.


    “I did an assay using RNA/DNA shield and it works perfect. The product has excellent reproducibility when stored at 4°C for up to a month.”

    - Maha AE. (University of Florida)

    “I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”

    - Laura T. (USDA)

    “We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4 or -20 for months, never saw any DNA degradation.”

    - Umberto P. (University of Pavia)


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