ChIP DNA Clean & Concentrator

D5205 / D5201


ChIP DNA Clean & Concentrator

D5205 / D5201

Cat # Name Size Price Quantity
D5205 ChIP DNA Clean & Concentrator (Capped Columns) 50 Preps $101.00
+ -
D5201 ChIP DNA Clean & Concentrator (Uncapped Columns) 50 Preps $96.00
+ -

Documents


DNA purification from any step in a ChIP assay.

Highlights


  • Two minute DNA clean-up from any step in a standard ChIP protocol.
  • DNA is ideal for PCR, arrays, DNA quantification, Southern blot analysis, sequencing, and other molecular applications.

Description


The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator provides a hassle-free method for the rapid purification and concentration of high-quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP.

Technical Specifications


Elution Volume ≥ 6 µl
Processing Time 2 minutes
Recovery 70-90% for DNA 50 bp - 10 kb
70% for DNA>10 kb
Size Range 50 bp - 23 kb
Storage All components should be stored at room temperature.

Citations


Chromatin immunoprecipitation assays were performed with A549 and Beas-2B cells using GR or FLAG antibodies. ChIPed DNA was purified with the ChIP DNA Clean and Concentrator kit from Zymo Research and the purified DNA was used to study the occupancy of KLF15 and GR at the GR-KLF15 response elements by qPCR analysis.

Sasse SK et al. (2013) The glucocorticoid receptor and KLF15 regulate gene expression dynamics and integrate signals through feed-forward circuitry. Mol Cell Biol. 33(11):2104-15.

Researchers performed a series of ARID1A chromatin immunoprecipitation (ChIP) experiments in primary ovarian surface epithelial cells. ChIPed DNA was purified with the ChIP DNA Clean & Concentrator Kit and purified DNA was used to determine the occupancy of ARID1A at the IL-6 promoter. These results, combined with PIK3CA expression data indicate that coexistence of ARID1A-PIK3CA mutations lead to IL-6 upregulation in their ovarian clear-cell carcinoma model.

Chandler RL et al. (2015) Coexistent ARID1A–PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signaling. Nat Commun.

Proteinase K/RNase-treated DNA fragments were purified using ChIP DNA Clean & Concentrator spin column and purified DNA was used to analyze the association of VDR and changes of histone H3 and H4 acetylation within the major regulatory modules of the CYP3A4 gene.

Pavek et al. (2010) Intestinal cell-specific vitamin D receptor (VDR)-mediated transcriptional regulation of CYP3A4 gene. Biochem Pharmacol. 79(2):277-87.

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Kit Components