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    Endura Tn5 Transposase

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    Endura Tn5 Transposase

    Cat # Name Size Price Quantity
    E1024-20 Endura Tn5 Transposase - Unloaded 20 µl (1 U/µl) $359.00
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    E1024-100 Endura Tn5 Transposase - Unloaded 100 µl (1 U/µl) $1,248.00
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    E1025-20 Endura Tn5 Transposase - Loaded 20 µl (1 U/µl) $429.00
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    E1025-100 Endura Tn5 Transposase - Loaded 100 µl (1 U/µl) $1,498.00
    - +
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    Highlights

    • Exceptional Stability: Optimized novel formulation for superior stability.
    • Batch-to-Batch Consistency: Enables highly reproducible assays and library preps.
    • Ready-to-Load: Easily generate transposomes or load with custom adapters with Unloaded format.
    • Ready-to-Tagment: Immediately tagment DNA for short-read sequencing library prep with Loaded format.
    Description & Documents Applications Specifications FAQ Related Products

    Documents

    Product Description

    EnduraTM Tn5 Transposase is hyperactive mutant of Tn5 Transposase with a proprietary formulation to confer superior stability, efficient tagmentation, and reliable consistency between experiments. EnduraTM Tn5 Transposase allows for easy, rapid, and consistent library preparation for next-generation libraries such as whole genome sequencing, shotgun sequencing, targeted sequencing, single-cell sequencing, and ATAC-Seq.

    Documents

    Applications

    • Whole Genome Sequencing (WGS) – Decode the entire DNA sequence of an organism’s genome to identify genetic variants, mutations, and structural variations.
    • Whole Exome Sequencing (WES) – Analyze the protein-coding regions of the genome to detect mutations linked to particular phenotypes, genetic disorders, and disease.
    • Assay for Transposase-Accessible Chromatin Sequence (ATAC-Seq) – Identify genome-wide chromatin accessibility and regulatory DNA elements in high resolution.
    • Targeted Sequencing – Focus on specific genes or genomic regions to detect known mutations, SNPs, and disease-related variants with accuracy.
    • Epigenomic Sequencing – Map DNA methylation and histone modifications to study gene expression regulation and epigenetic changes.
    • Metagenomic Sequencing – Analyze microbial DNA from environmental or host-associated samples to profile microbial diversity, taxonomy, and functional genes.
    • Single Cell Sequencing – Analyze gene expression, epigenetics, or genome variation at the individual cell level to uncover cellular heterogeneity.
    • RNA Sequencing – Capture and quantify the transcriptome to analyze gene expression, alternative splicing, and non-coding RNA profiles.
    • Transposome Studies – Examine transposable elements and mobile DNA to understand their role in genome evolution, stability, and regulation.

    Technical Specifications

    Activity Tn5 Transposase (1 U/µl)
    Format Liquid, 50% glycerol
    Concentration ~12.5 µM, ~1 mg/ml
    Optimum pH 7.5
    Optimum Temperature 55°C
    Storage Store at -20°C, For long term storage, store at -80°C.

    Resources

    Endura TM Tn5 Transposase is a hyperactive variant of Tn5 transposase with a novel formulation to confer superior stability. It can maintain its activity over many freeze-thaw cycles as well as incubation at room temperature.

    Endura TM Tn5 Transposase can be inactivated by addition of 10x Stop Buffer to a final concentration of 1x in your reaction, following by a 5 minute incubation at 75 °C.

    Yes, you can load Endura TM Tn5 Transposase with any custom adapter sequence, of any length, as long as the 3’ end contains 19-bp Mosaic-End sequence (5’ – CTGTCTCTTATACACATCT – 3’).

    EnduraTM Tn5 Transposase – Loaded has been pre-loaded with equal molar amounts of the P5 and P7 adapters by Illumina:

    P5 – 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’

    P7 – 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’

    Note: The mosaic end (ME) sequences are underlined.

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