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    Endura Tn5 Transposase | Unloaded

    Endura Tn5 Transposase is hyperactive mutant of Tn5 Transposase with a proprietary formulation to confer superior stability, efficient tagmentation, and consistent performance for NGS library preps and transposition experiments.

    Format

    Endura Tn5 Transposase - Unloaded

    Highlights

    • Exceptional Stability: Optimized novel formulation for superior stability.
    • Batch-to-Batch Consistency: Enables highly reproducible assays and library preps.
    • Ready-to-Load: Easily generate transposomes or load with custom adapters with Unloaded format.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

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    Innovated in California, Made in the USA

    Format

    Endura Tn5 Transposase - Unloaded

    Highlights

    • Exceptional Stability: Optimized novel formulation for superior stability.
    • Batch-to-Batch Consistency: Enables highly reproducible assays and library preps.
    • Ready-to-Load: Easily generate transposomes or load with custom adapters with Unloaded format.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    100% satisfaction guaranteed, read Our Promise

    Innovated in California, Made in the USA

    Cat # Name Size Price Quantity
    E1024-20 Endura Tn5 Transposase - Unloaded 20 µl (1 U/µl) $370.00
    - +
    E1024-100 Endura Tn5 Transposase - Unloaded 100 µl (1 U/µl) $1,285.00
    - +
    E1024-4-5 Endura Tn5 Dilution Buffer 5 mL $110.00
    - +

    Description

    Endura Tn5 Transposase is hyperactive mutant of Tn5 Transposase with a proprietary formulation to confer superior stability, efficient tagmentation, and reliable consistency between experiments. Endura Tn5 Transposase allows for easy, rapid, and consistent library preparation for next-generation libraries such as whole genome sequencing, shotgun sequencing, targeted sequencing, single-cell sequencing, and ATAC-Seq. 

    Learn More

    Performance

    Technical Specifications
    Activity Tn5 Transposase (1 U/µl)
    Format Liquid, 50% glycerol
    Concentration ~12.5 µM, ~1 mg/ml
    Optimum pH 7.5
    Optimum Temperature 55°C
    Storage Store at -20°C. For long term storage, store at -80°C.

    Resources

    Documents

    FAQ

    Endura Tn5 Transposase is a hyperactive variant of Tn5 transposase with a novel formulation to confer superior stability. It can maintain its activity over 10 freeze-thaw cycles as well as room temperature incubation.

    Yes, you can load Endura Tn5 Transposase with any custom adapter sequence or transposon DNA, of any length, as long as the 3’ end contains 19-bp Mosaic-End sequence (5’ – CTGTCTCTTATACACATCT – 3’).

    Endura Tn5 – Unloaded must first be loaded with adapters for the desired sequencing platform. Then the loaded Endura Tn5 transposome can be used to tagment the target DNA. From there, any polishing gap-fill, PCR, or clean-up can be done to prepare the final library.

    Endura Tn5 Transposase can be inactivated by addition of 10x Stop Buffer to a final concentration of 1x in your reaction, following by a 5-minute incubation at 75 °C.

    Applications

    • Whole Genome Sequencing (WGS) – Decode the entire DNA sequence of an organism’s genome to identify genetic variants, mutations, and structural variations.
    • Whole Exome Sequencing (WES) – Analyze the protein-coding regions of the genome to detect mutations linked to particular phenotypes, genetic disorders, and disease.
    • Assay for Transposase-Accessible Chromatin Sequencing (ATAC-Seq) – Identify genome-wide chromatin accessibility and regulatory DNA elements in high resolution.
    • Epigenomic Sequencing – Map DNA methylation and histone modifications to study gene expression regulation and epigenetic changes.
    • Metagenomic Sequencing – Analyze microbial DNA from environmental or host-associated samples to profile microbial diversity, taxonomy, and functional genes.
    • Single Cell Sequencing – Analyze gene expression, epigenetics, or genome variation at the individual cell level to uncover cellular heterogeneity.
    • RNA Sequencing – Capture and quantify the transcriptome to analyze gene expression, alternative splicing, and non-coding RNA profiles.
    • Transposome Studies – Examine transposable elements and mobile DNA to understand their role in genome evolution, stability, and regulation.
    Citations

    Endura Tn5 - Unloaded was loaded with customer adapters and used to tagment purified plant gDNA for tagmentation-based tag integration site sequencing (TTISS). The resulting libraries were sequenced on an AVITI sequencing platform.

    Muchenje, K.T., McCombe, C.L., Wang, Y. et al. Optimized R2 retroelement complexes for DNA insertion into plant genomes. Nat Biotechnol (2026).

    Endura Tn5 was used to tagment duplexed cDNA generated from vascular smooth muscle cell mRNA. The resulting libraries were sequenced on a NovaSeq X Plus sequencing platform.

    Yanzhi Wang, Cheng Luo, Songmao Wang, Li Li, Kailong Fu, Qing Rex Lyu, Single-cell lineage tracing unveils sex differences in VSMCs derived from the cardiac neural crest and second heart field, Vascular Pharmacology, Volume 163, 2026, 107611, ISSN 1537-1891.

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