EZ-96 DNA Methylation-Gold Kit

D5008 / D5007


EZ-96 DNA Methylation-Gold Kit

D5008 / D5007

Cat # Name Size Price Quantity
D5008 EZ-96 DNA Methylation-Gold Kit (Deep-well) 2 x 96 Rxns $391.00
+ -
D5007 EZ-96 DNA Methylation-Gold Kit (Shallow-Well) 2 x 96 Rxns $391.00
+ -

Documents


Complete bisulfite conversion of GC-rich DNA in less than 3 hours.

Highlights


  • High-throughput (96-well) bisulfite conversion of DNA in less than 3 hours. A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
  • Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate.
  • Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.

Description


The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to reduce incubation time and ensure conversion rates >99%. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

Technical Specifications


Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion >99%
Elution Volume ≥ 15 µl for Deep-well
≥ 30 µl for Shallow-well
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers
Input 500 pg - 2 µg of DNA.
Processing Time 3 hours
Recovery >75%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

Product FAQ


Q1: Tips for bisulfite primer design?

Q2: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?

Q3: Does bisulfite conversion only occur in a CpG context?

Q4: Which polymerase is recommended for amplification from bisulfite converted DNA?

Q5: What is the minimum DNA size that can be recovered?

Q6: How to quantify / visualize converted DNA?

Q7: What leads to poor conversion efficiency/ low yields?

Q8: How long is bisulfite converted DNA stable at -20 °C?

Citations


Reviews


“This protocol allows for bisulfite conversion in about 3 hours. This fast conversion is particularly relevant to our experiments, which allows us to PCR amplify and clone the products in 1 day, saving time without loss of quality.”

- Joseph M. (Sanford-Burnham Medical Research Institute)

“It makes gene specific DNA methylation analysis very simple and anyone with modest technical background can easily perform the experiment using this kit.”

- Murali B. (Centre for DNA Fingerprinting and Diagnostics)

Kit Components