EZ-96 DNA Methylation-Gold MagPrep
D5042 / D5043
- Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
- A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
- High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
|Applications||Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.|
|Elution Volume||≥ 25 µl|
|Equipment||Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.|
|Input||500 pg - 2 µg of DNA.|
|Processing Time||3 hours|
|Sample Source||Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.|
Q1: What leads to poor conversion efficiency/ low yields?
Q2: How to quantify / visualize converted DNA?
Q3: What is the minimum DNA size that can be recovered?
Q4: How long is bisulfite converted DNA stable at -20 °C?
Q5: Does bisulfite conversion only occur in a CpG context?
Q6: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?
Q7: Which polymerase is recommended for amplification from bisulfite converted DNA?
Q8: Tips for bisulfite primer design?
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