EZ-96 DNA Methylation-Gold MagPrep

D5042 / D5043

EZ-96 DNA Methylation-Gold MagPrep

Cat # Name Size Price Quantity
D5042 EZ-96 DNA Methylation-Gold MagPrep 4 x 96 Rxns. $745.20
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D5043 EZ-96 DNA Methylation-Gold MagPrep 8 x 96 Rxns. $1,152.00
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  • Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
  • A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
  • High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.

The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

For automation scripts and support, email automation@zymoresearch.com

Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99%
Elution Volume ≥ 25 µl
Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input 500 pg - 2 µg of DNA.
Processing Time 3 hours
Recovery > 70%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info

Poor conversion efficiency and low yields can be due to a variety of different experiment-specific conditions. Please contact Technical Support to discuss your specific experimental conditions and further troubleshoot with a product specialist.

Following bisulfite treatment of genomic DNA, nonmethylated cytosine residues are converted into uracil. The recovered DNA is typically A, U, and T-rich. The original base-pairing no longer exists. Instead, it is single-stranded with limited non-specific base-pairing at room temperature. The absorption coefficient at 260 nm resembles that of RNA. Use a value of 40 μg/ml for Ab260 = 1.0 when determining the concentration of the recovered bisulfite-treated DNA. To visualize, run converted DNA on agarose gel then chill the gel on ice for 30 minutes. The expected smear will be between 100-1500bp.

> 50 bp.

Converted DNA should be eluted in M-Elution Buffer to keep the converted DNA stable for long term storage. If stored properly for long term (<-20C), the samples should last longer than a month. Minimize freeze/thawing to keep the bisulfite converted DNA stable.

Bisulfite conversion will work regardless of context, so the kits are compatible with genomic DNA derived from plants and other species with high non-CpG methylation levels.

Leaving the desulphonation buffer on the column longer than recommended will cause more degradation and subsequently result in lower yields.

ZymoTaq DNA Polymerase has been specifically designed for use in bisulfite amplification reactions. ZymoTaq is available as a stand-alone polymerase (E2001/E2002), PreMix (E2003/E2004), and qPCR PreMix (E2054/E2055).

Cat # Name Size Price
D4100-5-8 MagBinding Beads 8 mL $101.20
D4100-5-16 MagBinding Beads 16 mL $182.60
D5041-6 M-Elution Buffer 40 ml $36.00
D5003-1 CT Conversion Reagent (96 Conversions) 1 Bottle $75.60
D5006-2 M-Dilution Buffer-Gold 7 ml $12.00
D5007-6 M-Elution Buffer 8 ml $12.00
D5040-4 M-Wash Buffer 72 ml $81.60
D5040-5 M-Desulphonation Buffer 80 ml $88.80
D5040-3 M-Binding Buffer 250 ml $126.00
D5006-6 M-Dissolving Buffer 1.2 ml $18.00
C2005 Conversion Plate w/ Cover Foil 2 Plates/Foils $10.00
C2002 Collection Plate 2 Plates $24.20
C2003 Elution Plate 2 Plates $20.90