EZ-96 DNA Methylation Kit

D5004 / D5003


EZ-96 DNA Methylation Kit

D5004 / D5003

Cat # Name Size Price Quantity
D5004 EZ-96 DNA Methylation Kit (Deep-Well) 2 x 96 Rxns $380.00
+ -
D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns $380.00
+ -

Documents


Streamlined bisulfite treatment of DNA.

Highlights


  • Streamlined, proven procedure for bisulfite conversion of DNA.
  • Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
  • Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

Description


The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc. Note: Catalog # D5004 is recommended for use with the Illumina Infinium MethylationEPIC BeadChip array.

Technical Specifications


Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion >99%
Elution Volume ≥ 15 µl for Deep-well
≥ 30 µl for Shallow-well
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input 500 pg - 2 µg of DNA
Processing Time 12-16 hours
Recovery >80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

Product FAQ


Q1: What leads to poor conversion efficiency/ low yields?

Q2: How to quantify / visualize converted DNA?

Q3: What is the minimum DNA size that can be recovered?

Q4: How long is bisulfite converted DNA stable at -20 °C?

Q5: Does bisulfite conversion only occur in a CpG context?

Q6: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?

Q7: Which polymerase is recommended for amplification from bisulfite converted DNA?

Q8: Tips for bisulfite primer design?

Citations


Kit Components