EZ-96 DNA Methylation MagPrep

D5040 / D5041


EZ-96 DNA Methylation MagPrep

D5040 / D5041

Cat # Name Size Price Quantity
D5040 EZ-96 DNA Methylation MagPrep 4 x 96 Rxns. $601.00
+ -
D5041 EZ-96 DNA Methylation MagPrep 8 x 96 Rxns. $960.00
+ -

Documents


EZ-96 DNA Methylation MagPrep

Highlights


  • Proven procedure for complete bisulfite conversion of DNA.
  • High-throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
  • Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

Description


The EZ-96 DNA Methylation MagPrep is a bisulfite conversion kit that features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

Technical Specifications


Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99%
Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input 500 pg - 2 µg of DNA
Processing Time 12-16 hours
Recovery > 70%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

Product FAQ


Q1: What leads to poor conversion efficiency/ low yields?

Q2: How to quantify / visualize converted DNA?

Q3: What is the minimum DNA size that can be recovered?

Q4: How long is bisulfite converted DNA stable at -20 °C?

Q5: Does bisulfite conversion only occur in a CpG context?

Q6: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?

Q7: Which polymerase is recommended for amplification from bisulfite-converted DNA?

Q8: Tips for bisulfite primer design?

Citations


Kit Components