Human Methylated & Non-methylated DNA Set

D5014 / D5014-1 / D5014-2


Human Methylated & Non-methylated DNA Set

D5014 / D5014-1 / D5014-2

Cat # Name Size Price Quantity
D5014 Human Methylated & Non-methylated DNA Set 5 µg/20 µl $435.00
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D5014-1 Human HCT116 DKO Non-Methylated DNA 5 µg/20 µl $238.00
+ -
D5014-2 Human HCT116 DKO Methylated DNA 5 µg/20 µl $238.00
+ -

Documents


Data Sheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Control for bisulfite conversion; DNA methylation quantitation

Highlights


  • Purified, non-methylated and methylated human DNA for use as negative and positive controls in methylation detection applications. Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
  • Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
  • Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion

Description


The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.

Technical Specifications


Concentration 250 ng/µl
Format Purified genomic DNA
Storage ≤ -20°C