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DNA/RNA Shield (50 ml)Cat#: R1100-50DNA/RNA Shield reagent is a DNA and RNA stabilization solution for nucleic acids in any biological sample. This DNA and RNA stabilization solution preserves the...
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Read The Zymo Research PromiseLoad N’ Go DNA/RNA Viral Kit
Cat # | Name | Size | Price | Quantity |
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Highlights
- Pre-filled 96-well reagent plate technology that offers multi-platform compatibility and reduces hands-on time by 75%.
- High-throughput, magnetic-bead based purification of viral DNA and RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, swab, fecal, and biopsy samples.
Documents
Product Description
Technical Specifications
Binding Capacity | Up to 5 µg DNA/RNA per reaction. |
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Elution Volume | Prefilled at 50 µl DNase/RNase-Free Water per well. |
Purity | DNA/RNA is ready for Nex-Gen Sequencing, RT/qPCR, etc. |
Sample Source | Plasma, serum, saliva, swab, urine, cell culture media, blood, cellular suspension, fecal sample or biopsy sample. |
Resources
Q1: How do I know if my automation instrument is compatible?
Examples of popular systems that are compatible include, but are not limited to:
- AllSheng Auto-Pure 96
- IsoPureTM 96
- KingFisherTM Flex
- KingFisherTM Apex
- Tecan Fluent®
- Hamilton Microlab®StarTM
- OpentronsTM OT-2
If you are unsure about compatibility, reach out to automation@zymoresearch.com for verification.
Q2: How do I get scripts for this kit on my automation platform?
Please contact automation@zymoresearch.com to send a request for scripts and additional reference material.
Q3: How to prevent RNA degradation?
Immediately collect and lyse fresh sample into a stabilization reagent, such as DNA/RNA Shield to ensure nucleic acid stability. Homogenized samples in DNA/RNA Shield can be stored frozen for later processing.
Q4: How to prevent low nucleic acid content and/or low sensitivity in downstream applications?
Incomplete deproteinization due to high-protein content in the sample (blood, plasma/serum, tissue, etc.):
- Increase the volume of DNA/RNA Shield to the sample
- Perform Proteinase K treatment (see Sample Preparation, page 5).
Increase eluate input:
- Titrate the DNA/RNA eluate for downstream applications (i.e., RT, qPCR).
Q5: How to address DNA contamination?
To remove DNA, use the RNA Clean & Concentrator MagBead Kit (Cat #R1082, sold separately):
- Use the included DNase I post-purification treatment
- Perform the RNA Clean & Concentrator MagBead clean-up protocol.
Q6: Can I still use DNA/RNA Shield if a precipitate has formed?
This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.
Cat # | Name | Size | Price | |
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R1200-25 | DNA/RNA Shield (2X Concentrate) | 25 ml | $70.00 | |
D3001-2-20 | Proteinase K w/ Storage Buffer Set | 20 mg | $49.90 | |
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