Quick-DNA/RNA Viral MagBead

R2140 / R2141 / R2140-E / R2141-E


Quick-DNA/RNA Viral MagBead

Cat # Name Size Price Quantity
R2140 Quick-DNA/RNA Viral MagBead 1 x 96 Preps $293.00
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R2141 Quick-DNA/RNA Viral MagBead 4 x 96 Preps $1,069.00
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R2140-E Quick-DNA/RNA Viral MagBead – DX 1 x 96 Preps $293.00
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R2141-E Quick-DNA/RNA Viral MagBead – DX 4 x 96 Preps $1,069.00
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Quick-DNA/RNA Viral MagBead

Highlights

  • High-throughput, magnetic-bead based purification of viral DNA and RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, swab, fecal and biopsy samples
  • High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
  • DNA/RNA Shield included for sample collection, inactivation and storage.
Description

Quick-DNA/RNA Viral MagBead kit is designed for high-throughput purification of viral DNA and/or RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies and swab and fecal samples stored in DNA/RNA Shield. The kit also features a buffer system that facilitates complete viral particle lysis for efficient nucleic acid isolation. Small (>50 nt) and large (>200 kb) DNA and RNA are bound to magnetic beads, washed and eluted. The isolated high-quality nucleic acids are ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.

Learn more about early viral detection

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Equipment Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually.
Purity RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Sample Source Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies and swab and fecal samples
Size Range 50 nt to ~200 kb
Yield 10 µg DNA/RNA binding capacity (per 20 µl MagBinding Beads), ≥50 µl elution volume

Q1: Is the addition of beta-mercaptoethanol necessary?

The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.

Q2: Is this kit compatible with samples stored in UTM/VTM?

Yes, these samples are compatible.

Q3: Will this kit isolate DNA?

Yes, this kit will co-purify some DNA.

Q4: Can DNase I treatment be performed?

Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).

Q5: Is it normal for the Viral RNA (DNA/RNA) Buffer to range in color between clear and yellow?

Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.

Q6: What is the expiration of the Viral RNA Buffer when beta-mercaptoethanol has been added?

The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.

Q7: Why are my columns/plate wells getting clogged?

In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.

Q8: Why am I seeing delayed/no amplification during RT/qPCR?

For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.




Cat # Name Size Price
R1200-125 DNA/RNA Shield (2X Concentrate) 125 ml $241.00
D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg $44.00
D4100-2-12 MagBinding Beads 12 ml $125.00
D4100-2-3 MagBinding Beads 3 ml $66.00
W1001-30 DNase/RNase-Free Water 30 ml $22.00
D7020-1-100 Viral DNA/RNA Buffer 100 ml $163.00
R2130-1-120 MagBead DNA/RNA Wash 1 120 ml $198.00
R2130-1-30 MagBead DNA/RNA Wash 1 30 ml $63.00
R2130-2-20 MagBead DNA/RNA Wash 2 20 ml $54.00
R2130-2-80 MagBead DNA/RNA Wash 2 80 ml $171.00