Quick-DNA Fecal/Soil Microbe Microprep

D6012


Quick-DNA Fecal/Soil Microbe Microprep

D6012

Cat # Name Size Price Quantity
D6012 Quick-DNA Fecal/Soil Microbe Microprep 50 Preps $216.00
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Documents


Extract high quality, inhibitor-free metagenomic DNA from feces, soil, water, etc.

Highlights


  • Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.

Description


The Quick-DNA Fecal/Soil Microbe Microprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 20 µl
Equipment Microcentrifuge, vortex, cell disruptor/pulverizer
Processing Volume ≤50 mg feces, ≤ 250 mg soil, ≤20 mg fungal/bacterial cells (wet weight)
Purity A260/A280 nm ≥1.8.
Sample Source Feces or soil
Sample Storage DNA stored at ≤ -20°C.
Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Type Total DNA
Yield ≤ 5 µg total DNA

Product FAQ


Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?

Q2: Are there any tips in optimzing bead beating conditions?

Q3: What is the purpose of Zymo-Spin II-µHRC step?

Q4: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q5: When can an RNase A treatment be implemented in the protocol?

Citations


Kit Components