Quick-DNA Fecal/Soil Microbe Microprep

D6012


Quick-DNA Fecal/Soil Microbe Microprep

D6012

Cat # Name Size Price Quantity
D6012 Quick-DNA Fecal/Soil Microbe Microprep 50 Preps $216.00
+ -

Documents


Datasheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Extract high quality, inhibitor-free metagenomic DNA from feces, soil, water, etc.

Highlights


  • Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.

Description


The Quick-DNA Fecal/Soil Microbe Microprep Kit is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 20 µl
Equipment Microcentrifuge, vortex, cell disruptor/pulverizer
Processing Volume ≤50 mg feces, ≤ 250 mg soil, ≤20 mg fungal/bacterial cells (wet weight)
Purity A260/A280 nm ≥1.8.
Sample Source Feces or soil
Sample Storage DNA stored at ≤ -20°C.
Size Range Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
Type Total DNA
Yield ≤ 5 µg total DNA

Product FAQ


Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?

Q2: Are there any tips in optimzing bead beating conditions?

Q3: What is the purpose of Zymo-Spin II-µHRC step?

Q4: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q5: When can an RNase A treatment be implemented in the protocol?

Kit Components