Quick-DNA Fecal/Soil Microbe Microprep
- Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
The Quick-DNA Fecal/Soil Microbe Microprep Kit is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 20 µl|
|Equipment||Microcentrifuge, vortex, cell disruptor/pulverizer|
|Processing Volume||≤50 mg feces, ≤ 250 mg soil, ≤20 mg fungal/bacterial cells (wet weight)|
|Purity||A260/A280 nm ≥1.8.|
|Sample Source||Feces or soil|
|Sample Storage||DNA stored at ≤ -20°C.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.|
|Yield||≤ 5 µg total DNA|