Quick-DNA Fungal/Bacterial Microprep Kit

D6007


Quick-DNA Fungal/Bacterial Microprep Kit

D6007

Cat # Name Size Price Quantity
D6007 Quick-DNA Fungal/Bacterial Microprep Kit 50 Preps $170.00
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Documents


Datasheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Easily isolate DNA from tough-to-lyse fungi and bacteria.

Highlights


  • Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.
  • Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
  • Simple: Fastest workflow (≤ 20 minutes).

Description


The Quick-DNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 10 µl
Equipment Microcentrifuge, vortex, cell disruptor/pulverizer
Processing Time ≤ 15 minutes
Processing Volume ≤20 mg fungi or bacteria (wet weight), 2x108 bacterial cells, 2x107 yeast cells, or 2x106 mammalian cells
Purity Typical A260/A280 & A260/A230 ≥ 1.8
Sample Source Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.
Size Range Capable of recovering genomic DNA sized fragments from up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered
Type Total DNA
Yield ≤ 5 µg total DNA

Product FAQ


Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?

Q2: Are there any tips in optimizing bead beating conditions?

Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q4: When can an RNase A treatment be implemented in the protocol?

Kit Components