Quick-DNA Fungal/Bacterial Miniprep Kit
- Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
- Simple: Fastest workflow (≤ 20 minutes).
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 35 µl|
|Equipment||Microcentrifuge, vortex, cell disruptor/pulverizer|
|Processing Time||≤ 15 minutes|
|Processing Volume||≤100 mg fungi or bacteria (wet weight), 109 bacterial cells, 108 yeast cells, or 107 mammalian cells|
|Purity||Typical A260/A280 & A260/A230 ≥ 1.8|
|Sample Source||Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.|
|Size Range||Capable of recovering genomic DNA sized fragments from up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered|
|Yield||≤ 25 µg total DNA|
Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?
Q2: Are there any tips in optimizing bead beating conditions?
Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Q4: When can an RNase A treatment be implemented in the protocol?
The ZR Fungal/Bacterial DNA MiniPrep was used to extract genomic DNA from B. neritina colonies for assessment of host genotype and symbiotic status by PCR. Analysis of the transcriptome suggests that symbiont-produced bryostatins are involved in reproduction of host B. neritina via PKC activation.Mathew M, et. al. (2016). Influence of symbiont-produced bioactive natural products on holobiont fitness in the marine bryozoan, Bugula neritina via protein kinase C (PKC). Mar. Biol. 163:44.
Epichloë myecelium genomic DNA isolated using the ZR Fungal/Bacterial DNA MiniPrep in a study identifying mutations in perA including a mutation in the C-terminal reductase domain resulted in some distinct species with nonfunctional peramine synthesis.Berry D, et. al. (2015) Disparate Independent Genetic Events Disrupt the Secondary Metabolism Gene perA in Certain Symbiotic Epichloë Species. Appl Environ Microbiol. 81(8):2797-2807.
DNA was isolated from various Lactobacillus species using the ZR Fungal/Bacterial DNA MiniPrep to characterize and sequence the different strains in cheese. This allows for greater identification of the relationship between human diet and the intestinal microbiome.dos Santos KMO, et. al. (2015) Artisanal Coalho cheeses as source of beneficial Lactobacillus plantarum and Lactobacillus rhamnosus strains. Dairy Science and Technology. 95(2):209-230.