Quick-DNA Plant/Seed Miniprep Kit
- Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 50 µl|
|Equipment||Microcentrifuge, vortex, cell disruptor/pulverizer|
|Processing Volume||≤ 150 mg|
|Purity||A260/A280 nm ≥1.8.|
|Sample Source||Leaves, stems, buds, flowers, fruit, seeds, etc.|
|Sample Storage||DNA stored at ≤ -20°C.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.|
|Yield||≤ 25 µg total DNA|
Q1: Can you provide a list of the tested plant species?
Q2: My lysate seems viscous. What is causing this to happen? How can I fix this?
Q3: Are there any tips in optimzing bead beating conditions?
Q4: What is the purpose of Zymo-Spin III-HRC step?
Q5: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Q6: When can an RNase A treatment be implemented in the protocol?
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