Quick-DNA Plant/Seed Miniprep Kit

D6020


Quick-DNA Plant/Seed Miniprep Kit

D6020

Cat # Name Size Price Quantity
D6020 Quick-DNA Plant/Seed Miniprep Kit 50 Preps $216.00
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Documents


Datasheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Inhibitor-free DNA isolated directly from plant and seed samples.

Highlights


  • Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.

Description


The Quick-DNA Plant/Seed Miniprep Kit is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 50 µl
Equipment Microcentrifuge, vortex, cell disruptor/pulverizer
Processing Volume ≤ 150 mg
Purity A260/A280 nm ≥1.8.
Sample Source Leaves, stems, buds, flowers, fruit, seeds, etc.
Sample Storage DNA stored at ≤ -20°C.
Size Range Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
Type Total DNA
Yield ≤ 25 µg total DNA

Product FAQ


Q1: Can you provide a list of the tested plant species?

Q2: My lysate seems viscous. What is causing this to happen? How can I fix this?

Q3: Are there any tips in optimzing bead beating conditions?

Q4: What is the purpose of Zymo-Spin III-HRC step?

Q5: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q6: When can an RNase A treatment be implemented in the protocol?

Kit Components