Quick-DNA Tissue/Insect Miniprep Kit

D6016


Quick-DNA Tissue/Insect Miniprep Kit

D6016

Cat # Name Size Price Quantity
D6016 Quick-DNA Tissue/Insect Miniprep Kit 50 Preps $170.00
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Documents


Datasheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Isolate DNA from tough-to-lyse tissues and insects.

Highlights


  • Simple and efficient isolation of DNA from insects, including mosquitoes, bees, lice, ticks, and D. melanogaster .
  • Compatible with tough-to-lyse tissues from other organisms.

Description


The Quick-DNA Tissue/Insect Miniprep Kit is designed for the simple and rapid isolation of DNA (e.g., genomic, viral, mitochondrial) from fresh, frozen, or stored insect specimens including mosquitoes, bees, lice, ticks, and D. melanogaster . The procedure is easy and can be completed in minutes: samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. The DNA is then isolated and purified using our Zymo-Spin column technology and is ideal for downstream molecular-based applications including PCR, array, genotyping, etc. The procedure is compatible with mammalian tissues, whole blood, and cultured cells.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 35 µl
Equipment Microcentrifuge, vortex, cell disruptor/pulverizer
Purity A260/A280 nm ≥1.8.
Sample Source Small amounts (n ≥ 1 and ≤ 50 mg) of fresh, frozen, or stored insects. Also, compatible with fresh or frozen mammalian tissues, as well as cultured cells and whole blood.
Sample Storage DNA stored at ≤ -20°C.
Size Range Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
Type Total DNA
Yield ≤ 25 µg total DNA

Product FAQ


Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?

Q2: Are there any tips in optimzing bead beating conditions?

Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q4: When can an RNase A treatment be implemented in the protocol?

Kit Components