ssDNA/RNA Clean & Concentrator
D7010 / D7011
- Quick and Reliable: 10 minute clean-up and concentration of ssDNA/RNA (17-200 nt).
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- Clean and Pure: ssDNA/RNA ready for downstream applications like PCR, RT-qPCR, etc.
|Purity||DNA/RNA is ready for subsequent molecular methods including PCR, RT-PCR, hybridization, etc.
A260/280 > 1.8
A260/230 > 1.8
|Sample Source||Mixtures of double and single stranded DNA and RNA species with single stranded fragments 17 to 200 nucleotides (e.g., short transcripts, probes, primers)|
|Size Range||DNA and/or RNA 17-200 nt|
|Yield||10 µg DNA/RNA|
The ssDNA/RNA Clean & Concentrator was used to separate single-stranded RNA from total RNA for gene expression analysis in Diaphorina citri.Hajeri, S et al. Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing). Journal of Biotechnology. 2014.
Biotinylated CD4 ssDNA aptamers were mixed with streptavidin and this conjugate was purified with the ssDNA/RNA Clean & Concentrator to remove free streptavidin. Functionality of aptamer polymers showed significant blocking (up to 70% inhibition) of binding of viral gp120 to CD4 expressing cells.Zhao, N et al. Blocking interaction of viral gp120 and CD4-expressing T cells by single-stranded DNA aptamers. The International Journal of Biochemistry & Cell Biology. 2014.
In vitro transcribed biotinylated transcripts were purified using the ssDNA/RNA Clean & Concentrator for biotin pull-down assay and subsequent western blotting of the RNA-protein complexes.Kuwano, Y et al. Transformer 2β and miR-204 regulate apoptosis through competitive binding to 3’ UTR of BCL2 mRNA. Cell Death & Differentiation. 2015.