Universal Methylated DNA Standard

D5011 / D5012


Universal Methylated DNA Standard

Cat # Name DNA Type Price Quantity
D5011 Universal Methylated Human DNA Standard Human $207.00
- +
D5012 Universal Methylated Mouse DNA Standard Mouse $207.00
- +

Documents

Universal Methylated DNA Standard

Highlights
  • Purified, methylated human DNA (D5011) and methylated mouse DNA (D5012) for use as controls to assess bisulfite conversion efficiency. Compatible with the EZ DNA Methylation family of products and other bisulfite conversion protocols.
  • All cytosines within a CpG dinucleotide context have been enzymatically methylated by M. SssI methyltransferase.
  • Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
Description

The Universal Methylated DNA Standards are designed to be used as positive controls to assess the efficiency of DNA bisulfite conversion in human and mouse models. The control DNA can be run in parallel with samples to monitor the bisulfite conversion reaction. The primer sets are designed to amplify a fragment of the supplied DNA from the respective species following bisulfite treatment.

Concentration 250 ng/µl
Storage Store DNA standard and primers at ≤ -20°C for up to 12 months. Prolonged storage should be ≤ -70°C.
Supplemental Info

The sample is derived from human male brain tissue.

All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).