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    Universal Methylated DNA Standard

    Highlights

    • Purified, methylated human DNA (D5011) and methylated mouse DNA (D5012) for use as controls to assess bisulfite conversion efficiency. Compatible with the EZ DNA Methylation family of products and other bisulfite conversion protocols.
    • All cytosines within a CpG dinucleotide context have been enzymatically methylated by M. SssI methyltransferase.
    • Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
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    Highlights

    • Purified, methylated human DNA (D5011) and methylated mouse DNA (D5012) for use as controls to assess bisulfite conversion efficiency. Compatible with the EZ DNA Methylation family of products and other bisulfite conversion protocols.
    • All cytosines within a CpG dinucleotide context have been enzymatically methylated by M. SssI methyltransferase.
    • Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Cat # Name DNA Type Price Quantity
    D5011 Universal Methylated Human DNA Standard Human $219.60
    - +
    D5012 Universal Methylated Mouse DNA Standard Mouse $219.60
    - +

    Description

    The Universal Methylated DNA Standards are designed to be used as positive controls to assess the efficiency of DNA bisulfite conversion in human and mouse models. The control DNA can be run in parallel with samples to monitor the bisulfite conversion reaction. The primer sets are designed to amplify a fragment of the supplied DNA from the respective species following bisulfite treatment.

    Performance

    Technical Specifications
    Concentration 250 ng/µl
    Storage Store DNA standard and primers at ≤ -20°C for up to 12 months. Prolonged storage should be ≤ -70°C.

    Resources

    Documents

    FAQ

    The sample is derived from human male brain tissue.

    All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).

    Citations

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