Zymo-Seq RiboFree Total RNA Library Kit
R3000 / R3000S
- The Fastest & Easiest Total RNA-Seq Library Kit: Prepare stranded, RiboFree libraries from total RNA in 3.5 hours.
- Compatible with Any Sample: Probe-free technology depletes rRNA & Globin from any RNA source.
- The Most Accurate: Eliminate bias from rRNA depletion.
|Equipment Required||Thermocycler, magnet stand*, and microcentrifuge.
*for a complimentary magnet stand, please contact firstname.lastname@example.org
|Input Quality||Ensure RNA A260/A280 and A260/A230 ratios are ≥ 1.8, DNA-free, and PCR inhibitor-free for high-fidelity cDNA transcription and depletion|
|Maximum Input||5 µg|
|Minimum Input||100 ng|
|Processing Time||As little as 3.5 hours (RNA to indexed library)|
|Recommended Input||500 ng|
|Sample Input Material||RNA from any species|
|Sequencing||Libraries are stranded and compatible with all Illumina® sequencing platforms. The Read 1 sequence will be antisense to the RNA molecule of origin.|
Q1: Do you sell the ribo depletion separately?
Q2: Will this kit work with prokaryotes?
Q3: My sample is lower than 100 ng, will this kit still work?
“I think this kit is faster and simpler to do than the Truseq RNA library prep kit. It was easy enough that I tried it once and got a nice library prep, with a high concentration and I did not make any mistakes in my first try! I will definitely buy this kit and recommend it to others without a doubt.”
- Rafael (UC Berkeley)
“This RNA Library Kit was easy to use and recovered good efficiency.”
- Veronica L (CICY Mexico)
“…very satisfied. It was easy and the depletion was effective.”