Zymo-Seq RiboFree Universal cDNA Kit
R3001
Zymo-Seq RiboFree Universal cDNA Kit
Cat # | Name | Size | Price | Quantity |
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Documents

Highlights
- The Easiest Kit: Prepare RiboFree cDNA from total RNA in as little as 5 pipetting steps.
- Compatible with Any Sample: Probe-free technology depletes rRNA from any RNA source.
- The Most Accurate: Eliminate bias from rRNA depletion.
Description
Equipment Required | Thermocycler, magnet stand, and microcentrifuge |
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Input Quality | Ensure RNA A260/A280 and A260/A230 ratios are ≥ 1.8, DNA-free, and PCR inhibitor-free for high-fidelity cDNA transcription and depletion |
Maximum Input | 5 µg |
Minimum Input | 100 ng |
Processing TIme | As little as 1.5 hours (RNA to single-stranded cDNA) |
Recommended Input | 500 ng |
Sample Input Material | RNA |
Q1: Does this kit yield RNA?
No, the final product is single-stranded cDNA.
Q2: Is this part of a library prep kit?
Yes, this is included in the Zymo-Seq RiboFree Total RNA Library Kit
Q3: What library prep kit is this compatible with?
This is compatible with any library prep method that uses single-stranded DNA as an input.
Q4: Will this kit work with prokaryotes?
Yes, this kit will work with any organism.
Q5: My sample is lower than 100 ng, will this kit still work?
Inputs lower than 100 ng will require a longer incubation time during RiboFree Universal Depletion, and may show reduced rRNA depletion efficiency. Please refer to Appendix B for suggestions.
Cat # | Name | Size | Price | |
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R3004-1-6 | Zymo-Seq Wash Buffer | 6 mL | $18.00 | |
3DP-1002 | PCR Strip MagStand | 1 unit | $55.00 | |
D3004-4-1 | DNA Elution Buffer | 1 ml | $12.10 | |
D4084-4-10 | Select-a-Size MagBead (10mL) | 10 ml | $401.50 | |
D4084-4-50 | Select-a-Size MagBead (50mL) | 50 ml | $909.70 | |
W1001-1 | DNase/RNase-Free Water | 1 ml | $11.00 | |