Zymo-Seq RiboFree Universal cDNA Kit


Zymo-Seq RiboFree Universal cDNA Kit

Cat # Name Size Price Quantity
R3001 Zymo-Seq RiboFree Universal cDNA Kit 12 Preps $495.00
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  • The Easiest Kit: Prepare RiboFree cDNA from total RNA in as little as 5 pipetting steps.
  • Compatible with Any Sample: Probe-free technology depletes rRNA from any RNA source.
  • The Most Accurate: Eliminate bias from rRNA depletion.

The Zymo-Seq RiboFree Universal cDNA Kit produces first-strand cDNA, and is the easiest rRNA depletion kit available. The RiboFree Universal Depletion is compatible with RNA from all biological sample types, tissues (including whole blood), and does not require organism-specific probes to deplete rRNA. The RiboFree single-stranded cDNA produced by the kit can be used for qPCR detection, RNA-Seq library prep, and any other downstream analysis. In contrast to poly(A) enrichment methods, the RiboFree Universal cDNA Kit produces single-strand cDNA from nearly all RNA biotypes, including full-length mRNA, mRNA with degraded poly(A) tails, long-noncoding RNA (lncRNA), intronic RNA, nucleolar RNA, etc. The cost-effective Zymo-Seq RiboFree Universal cDNA Kit has been optimized to reduce hands-on time, and the probe-free method eliminates transcriptome profile bias compared to popular rRNA depletion and poly(A) enrichment kits.

Equipment Required Thermocycler, magnet stand, and microcentrifuge
Input Quality Ensure RNA A260/A280 and A260/A230 ratios are ≥ 1.8, DNA-free, and PCR inhibitor-free for high-fidelity cDNA transcription and depletion
Maximum Input 5 µg
Minimum Input 100 ng
Processing TIme As little as 1.5 hours (RNA to single-stranded cDNA)
Recommended Input 500 ng
Sample Input Material RNA

No, the final product is single-stranded cDNA.

Yes, this is included in the Zymo-Seq RiboFree Total RNA Library Kit

This is compatible with any library prep method that uses single-stranded DNA as an input.

Yes, this kit will work with any organism.

Inputs lower than 100 ng will require a longer incubation time during RiboFree Universal Depletion, and may show reduced rRNA depletion efficiency. Please refer to Appendix B for suggestions.

Cat # Name Size Price
R3004-1-6 Zymo-Seq Wash Buffer 6 mL $18.00
3DP-1002 PCR Strip MagStand 1 unit $55.00
D3004-4-1 DNA Elution Buffer 1 ml $12.10
D4084-4-10 Select-a-Size MagBead (10mL) 10 ml $401.50
D4084-4-50 Select-a-Size MagBead (50mL) 50 ml $909.70
W1001-1 DNase/RNase-Free Water 1 ml $11.00