Zymo-Seq RiboFree Universal cDNA Kit

R3001


Zymo-Seq RiboFree Universal cDNA Kit

R3001

Cat # Name Size Price Quantity
R3001 Zymo-Seq RiboFree Universal cDNA Kit 12 Preps $495.00
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Zymo-Seq RiboFree Universal cDNA Kit

Highlights


  • The Easiest Kit: Prepare RiboFree cDNA from total RNA in as little as 5 pipetting steps.
  • Compatible with Any Sample: Probe-free technology depletes rRNA & globin from any RNA source.
  • The Most Accurate: Eliminate bias from rRNA depletion.

Description


The Zymo-Seq RiboFree Universal cDNA Kit produces first-strand cDNA, and is the easiest rRNA and globin depletion kit available. The RiboFree Universal Depletion is compatible with RNA from all biological sample types, tissues (including whole blood), and does not require organism-specific probes to deplete rRNA and globin. The RiboFree single-stranded cDNA produced by the kit can be used for qPCR detection, RNA-Seq library prep, and any other downstream analysis. In contrast to poly(A) enrichment methods, the RiboFree Universal cDNA Kit produces single-strand cDNA from nearly all RNA biotypes, including full-length mRNA, mRNA with degraded poly(A) tails, long-noncoding RNA (lncRNA), intronic RNA, nucleolar RNA, etc. The cost-effective Zymo-Seq RiboFree Universal cDNA Kit has been optimized to reduce hands-on time, and the probe-free method eliminates transcriptome profile bias compared to popular rRNA depletion and poly(A) enrichment kits.

Technical Specifications


Equipment Required Thermocycler, magnet stand (free at checkout), and microcentrifuge
Input Quality Ensure RNA A260/A280 and A260/A230 ratios are ≥ 1.8, DNA-free, and PCR inhibitor-free for high-fidelity cDNA transcription and depletion
Maximum Input 5 µg
Minimum Input 100 ng
Processing TIme As little as 1.5 hours (RNA to single-stranded cDNA)
Recommended Input 500 ng
Sample Input Material RNA

Product FAQ


Q1: Does this kit yield RNA?

Q2: Is this part of a library prep kit?

Q3: What library prep kit is this compatible with?

Q4: Will this kit work with prokaryotes?

Q5: My sample is lower than 100 ng, will this kit still work?

Kit Components