- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 50 µl DNA Elution Buffer or DNase free water|
|Equipment||Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).|
|Processing Volume||Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x109 bacterial, 2x108 yeast, or 2x107 mammalian cells)|
|Purity||High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8|
|Sample Source||Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.|
|Sample Storage||Eluted DNA should be stored at ≤ -20°C.|
|Size Range||Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.|
|Yield||Up to 25 µg total DNA can be eluted into ≥50 µl|
|D4300-1-40||ZymoBIOMICS Lysis Solution||40 ml||$40.00|
|D4300-2-100||ZymoBIOMICS DNA Binding Buffer||100 ml||$138.00|
|D4300-3-50||ZymoBIOMICS DNA Wash Buffer 1||50 ml||$46.00|
|D4300-4-60||ZymoBIOMICS DNA Wash Buffer 2||60 ml||$32.00|
|D4300-7-30||ZymoBIOMICS HRC Prep Solution||30 ml||$98.00|
|D4302-5-1||ZymoBIOMICS DNase/RNase Free Water||1 ml||$10.00|
|D4302-5-10||ZymoBIOMICS DNase/RNase Free Water||10 ml||$28.00|
|C1057-50||Zymo-Spin III-F Filters||50 Pack||$59.00|
|C1058-50||Zymo-Spin III-HRC Filters||50 Pack||$108.00|
|S6012-50||ZR BashingBead Lysis Tubes (0.1 & 0.5 mm)||50 Tubes||$101.00|
|C1078-50||Zymo-Spin IICR Columns||50 Pack||$55.00|