- Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.
- Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc.
|Applicable For||Cleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc.|
|Elution Volume||50 - 200 µl|
|Purity||High quality, enzymatic-reaction ready DNA/RNA products.|
|Sample Source||DNA/RNA samples contaminated with enzymatic reaction inhibitors.|
|Sample Storage||Eluted DNA/RNA can be used immediately or stored at ≤ -20°C.|
|Type||DNA and RNA|
|Yield||50 - 90% recovery|
Q1: What is the smallest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?
Q2: What is the largest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?
Q3: Can I centrifuge at a lower speed than 3,500 x g for the 96-well kit?
The OneStep PCR Inhibitor Removal Kit was used to purify total RNA extracted from B. neritina colonies for sequence and phylogenetic studies. Analysis of the transcriptome suggests that symbiont-produced bryostatins are involved in reproduction of host B. neritina via PKC activation.Mathew M, et. al. (2016). Influence of symbiont-produced bioactive natural products on holobiont fitness in the marine bryozoan, Bugula neritina via protein kinase C (P
“The ease. Holy cow, this thing was so freaking simple compared to similar products”
- Christian H. (University of Georgia)
“It was VERY fast and yielded the intended results...PCR products from previously non-amplifiable DNA.”