ZymoBIOMICS RNA Miniprep Kit
- Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
- Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms.
The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc.
DNase I included.
|Equipment||Microcentrifuge, vortex, cell disrupter (recommended)|
|Purity||RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.|
|Sample Source||Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.|
|Size Range||Total RNA ≥17 nt|
|Yield||100 µg RNA (binding capacity), ≥50 µl (elution volume)|
Q1: What is the purpose of the Zymo-Spin III-HRC Step?
Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. The Zymo-Spin III-HRC is designed to remove these PCR inhibitors and does not bind DNA/RNA. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).
Q2: What do I do if I notice precipitation during ethanol addition?
Sample overloading can cause nucleic acids to precipitate and crash out of solution, reducing yields. Use less sample input to avoid precipitation.
Q3: What do I do if there is foaming of sample during homogenization?
This is a normal occurrence as DNA/RNA Shield contains detergents. To reduce foam, centrifuge at 12,000 x g in 1 minute intervals before transferring the supernatant.
|D4300-7-30||ZymoBIOMICS HRC Prep Solution||30 ml||$107.80|
|W1001-30||DNase/RNase-Free Water||30 ml||$24.20|
|R1100-50||DNA/RNA Shield||50 ml||$68.00|
|R1003-3-24||RNA Wash Buffer||24 ml||$64.90|
|C1006-50-G||Zymo-Spin IIICG Columns||50 Pack||$60.50|
|R1060-2-25||RNA Prep Buffer||25 ml||$44.00|
|C1001-50||Collection Tubes||50 Pack||$16.50|
|C1058-50||Zymo-Spin III-HRC Filters||50 Pack||$118.80|
|E1010-1-4||DNA Digestion Buffer||4 mL||$16.50|
|S6012-50||ZR BashingBead Lysis Tubes (0.1 & 0.5 mm)||50 Tubes||$111.10|