ZymoBIOMICS Spike-in Control II (Low Microbial Load)
D6321 / D6321-10
- Absolute Quantification: Enables cell number measurements from low bacterial load samples using Next-Gen Sequencing.
- In situ Quality Control: Ensures each sample is quantified accurately.
- Log Abundance Distribution: Three microbes alien to the human microbiome in log-distribution from 103 to 105 cells.
ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. They represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.
|16S rRNA genes||https://s3.amazonaws.com/zymo-files/BioPool/D6321.refseq.zip|
|Biosafety||This product is not biohazardous as microbes have been fully inactivated.|
|Impurity Level||< 0.01% foreign microbial DNA|
|Storage Solution||DNA/RNA Shield™|