ZymoBIOMICS Spike-in Control II (Low Microbial Load)

D6321 / D6321-10


ZymoBIOMICS Spike-in Control II (Low Microbial Load)

D6321 / D6321-10

Cat # Name Size Price Quantity
D6321 ZymoBIOMICS Spike-in Control II (Low Microbial Load) 25 Preps $98.00
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D6321-10 ZymoBIOMICS Spike-in Control II (Low Microbial Load) 250 Preps $499.00
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Documents

ZymoBIOMICS Spike-in Control II (Low Microbial Load)

Highlights

  • Absolute Quantification: Enables cell number measurements from low bacterial load samples using Next-Gen Sequencing.
  • In situ Quality Control: Ensures each sample is quantified accurately.
  • Log Abundance Distribution: Three microbes alien to the human microbiome in log-distribution from 103 to 105 cells.

Description

ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. They represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.

Technical Specifications

16S rRNA genes https://s3.amazonaws.com/zymo-files/BioPool/D6321.refseq.zip
Biosafety This product is not biohazardous as microbes have been fully inactivated.
Impurity Level < 0.01% foreign microbial DNA
Sample Storage -80°C
Storage Solution DNA/RNA Shield™