Understanding RNA-Seq and Ribosomal RNA Depletion

All NGS experiments utilizing the Illumina sequencing platform require the preparation of a sequencing library. In this video, learn why this process is necessary and how it enables sequencing on the Illumina platform through solid-phase or bridge PCR. Following that, we discuss some of the specific challenges with RNA-Seq library preparation related to the high abundance of ribosomal RNA sequences in nearly all sample types.


  • Library preparation
  • Understanding the role of adapters
  • Solid phase/bridge PCR within a flow cell
  • Pros and cons of different methods for rRNA depletion

Featured RNA-Seq Kit:

In the video we discussed the fastest and easiest total RNA-Seq library prep kit. Discover how simple library prep is using the Zymo-Seq RiboFree Total RNA Library Kit. Generate stranded, rRNA-depleted libraries in as little as 3.5 hours with 60% less pipetting than the TruSeq® Stranded Total RNA Library Prep Kit.

The RiboFree Universal Depletion allows researchers to use one kit to remove rRNA from any organism. Additionally, this new probe-free technology virtually eliminates bias caused by rRNA depletion compared to other probe-based methods, such as Ribo-Zero™.

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