|Applicable For||Next-Generation Sequencing, qPCR, microarray.|
|Reagent Storage||Ambient temperature => 2 years|
|Sample Collection||10% (v/v or w/v)|
|Sample Source||Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.|
|Sample Stability||RNA: Ambient temperature (4°C- 25°C) >1 month
DNA: Ambient temperature (4°C- 25°C) >2 years
DNA & RNA: Frozen (<-20°C): Indefinitely
Q1: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Q2: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
Q3: How to collect solid tissues in DNA/RNA Shield?
Q4: Can I FACS sort directly into DNA/RNA Shield?
Q5: How long can samples be stored frozen in DNA/RNA Shield?
Q6: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
Q7: What sample types is DNA/RNA Shield suitable for?
Q8: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
DNA/RNA Shield was used to store water collected onto filters and total RNA was isolated. A detailed metatranscriptomic analysis of the microbial communities points towards differing metabolic cycles occurring in anoxic and saline habitats in upper and deep sea regions respectively.Pachiadaki MG, et. al. (2014). Unveili
FACS sorted cells derived from disassociated 3D tissue matrix were collected in DNA/RNA Shield for downstream RNA-seq. Transcriptome analyses revealed that modifying crosslinking of hydrogels can optimize expression patterns to correlate with specific brain regions in an engineering 3D neural tissue.Tekin, H et al. Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Nature Biomedical Engineering. 2018.
DNA/RNA Shield was used to collect biomass samples from bench-scale microbial fuel cells. After bead-beating homogenization, the supernatant was used for automated DNA and RNA extraction on a Promega Maxwell Magnetic Particle Processor with the Maxwell 16 LEV Blood DNA and 16 LEV simplyRNA Blood kits.Chen, S et al. Methane-driven microbial fuel cells recover energy and mitigate dissolved methane emissions from anaerobic effluents. Environmental Science: Water Research & Technology. 2018.
“I did an assay using RNA/DNA shield and it works perfect. The product has excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4 or -20 for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)