|Applicable For||Next-Generation Sequencing, qPCR, microarray|
|Device Specs||Evacuated 16 mm x 100 mm blood collection tubes pre-filled with DNA/RNA Shield (6 mL)|
|Device Storage||Ambient temperature => 2 years|
|Sample Collection||3 mL blood draw|
|Sample Source||Whole blood (fresh or anti-coagulated)|
|Sample Stability||RNA: Ambient temperature (4°C-25°C) > 1 month
DNA: Ambient temperature (4°C-25°C) > 2 years
DNA & RNA: Frozen (< -20°C): Indefinitely
Q1: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Q2: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
Q3: How to collect solid tissues in DNA/RNA Shield?
Q4: Can I FACS sort directly into DNA/RNA Shield?
Q5: How long can samples be stored frozen in DNA/RNA Shield?
Q6: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
Q7: What sample types is DNA/RNA Shield suitable for?
Q8: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Mouse blood in EDTA was stored in DNA/RNA Shield (2X concentrate) and genomic DNA was extracted for reduced representation bisulfite sequencing (RRBS). Researchers were able to show that mammalian DNA methylomes are characterized by CpG sites that can represent an organism’s biological age.Petkovich, DA et al. Using DNA Methylation Profiling to Evaluate Biological Age and Longevity Interventions. Cell Metabolism. 2017.
West Nile virus positive plasma samples were inactivated in DNA/RNA Shield and resulting viral RNA was detected and identified by qRT-PCR. Phylogenetic comparisons between human and mosquito-derived strains were closely related but not identical which indicates co-circulation in a confined area.Kolodziejek J, et. al. (2015). West Nile Virus Positive Blood Donation and Subsequent Entomological Investigation, Austria, 2014. PLoS One. 10(5).
DNA/RNA Shield was used to preserve RNA in plasma samples positive for Dengue and Chikungunya virus. Extracted total RNA was used for downstream sequencing for genotyping and phylogenetic analysis. Results revealed two serotypes (DENV-2 and DENV-3) along with CHIKV infection representing the first documented co-infection.Phommanivong V, et. al. (2016). Co-circulation of the dengue with chikungunya virus during the 2013 outbreak in the southern part of Lao PDR. Tropical Medicine and Health. 44:24.
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)