Successfully Added to Cart
Customers also bought...
-
DNA/RNA Shield (50 ml)Cat#: R1100-50DNA/RNA Shield reagent is a DNA and RNA stabilization solution for nucleic acids in any biological sample. This DNA and RNA stabilization solution preserves the... -
DNA/RNA Shield SafeCollect Swab Collection Kit (1 ml fill) (1 collection kit)Cat#: R1160The DNA/RNA Shield SafeCollect Swab Collection Kit is a user-friendly collection kit for stabilizing the nucleic acid content of samples collected with a swab. DNA/RNA...
Highlights
- Quick (5 minute) clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
- Unique spin column for low volume elution of ultra-pure, high-yield DNA
- Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
Highlights
- Quick (5 minute) clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
- Unique spin column for low volume elution of ultra-pure, high-yield DNA
- Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1102-25 | Zymo-Spin IIC-XL Columns | 25 Pack | $39.70 | |
| D5201-1-50 | ChIP DNA Binding Buffer | 50 ml | $39.20 | |
| C1001-1000 | Collection Tubes | 1000 Pack | $105.10 | |
| C1001-500 | Collection Tubes | 500 Pack | $60.70 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.50 | |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $11.60 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $38.50 | |
| D3004-4-10 | DNA Elution Buffer | 10 ml | $16.40 | |
| D3004-4-1 | DNA Elution Buffer | 1 ml | $12.90 | |
| D3004-4-4 | DNA Elution Buffer | 4 ml | $11.60 | |
Description
Performance
Technical Specifications
| Applicable For | Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application |
|---|---|
| Elution Volume | ≥ 35 µl of DNA Elution Buffer |
| Equipment | Microcentrifuge |
| Purity | A260/A280 > 1.8, A260/A230 > 1.8 |
| Sample Source | Enzymatic reactions or impure preparations containing genomic DNAs |
| Sample storage | Eluted DNA can be used immediately or stored at ≤ -20°C |
| Size Range | 50 bp to 200 kb |
| Yield | Recovery of DNA ranges from 70 - 95% |
Resources
Documents
FAQ
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.
The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
We recommend no more than 5 times as binding efficiency might decrease.
Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
Citations
Need help? Contact Us