Quick-DNA Tissue/Insect Microprep Kit
- Simple and efficient isolation of DNA from insects, including mosquitoes, bees, lice, ticks, and D. melanogaster .
- Compatible with tough-to-lyse tissues from other organisms.
The Quick-DNA Tissue/Insect Microprep Kit is an insect DNA extraction kit designed for the simple and rapid isolation of DNA (e.g., genomic, viral, mitochondrial) from fresh, frozen, or stored insect specimens including mosquitoes, bees, lice, ticks, and D. melanogaster . The procedure is easy and can be completed in minutes: samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. The DNA is then isolated and purified using our Zymo-Spin column technology and is ideal for downstream molecular-based applications including PCR, array, genotyping, etc. The procedure is compatible with mammalian tissues, whole blood, and cultured cells.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 10 µl|
|Equipment||Microcentrifuge, vortex, cell disruptor/pulverizer|
|Purity||A260/A280 nm ≥1.8.|
|Sample Source||Small amounts (n ≥ 1 and ≤ 10 mg) of fresh, frozen, or stored insects. Also, compatible with fresh or frozen mammalian tissues, as well as cultured cells and whole blood.|
|Sample Storage||DNA stored at ≤ -20°C.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.|
|Yield||≤ 5 µg total DNA|
Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?
Q2: Are there any tips in optimzing bead beating conditions?
Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Q4: When can an RNase A treatment be implemented in the protocol?
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