Quick-RNA Viral 96 Kit
R1040 / R1041 / R1040-E / R1041-E
Quick-RNA Viral 96 Kit
- Quick, high-throughput (96-well spin plate) purification of viral RNA from plasma, serum, CSF, cell culture media, cellular suspensions, urine, blood, saliva, swab, fecal, etc.
- Omits the use of organic denaturants and proteases.
- High-quality viral RNA is ready for RT-PCR, sequencing, etc.
The Quick-RNA Viral 96 Kit provides for rapid, large scale isolation of viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield. DNA/RNA Shield ensures nucleic acid stability during sample storage/transport at ambient temperatures. The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the matrix in each well of the spin-plate, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.
|Equipment||Centrifuge with 96-well plate carrier|
|Purity||RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.|
|Registration Status||CE-IVD certified (R1040-E, R1041-E)|
|Sample Source||Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab and fecal samples|
|Size Range||50 nt to ~200 kb|
|Yield||Spin plate, 10 µg RNA (binding capacity), ≥10 µl (elution volume)|
Q1: Is the addition of beta-mercaptoethanol necessary?
The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.
Q2: Is this kit compatible with samples stored in UTM/VTM?
Yes, these samples are compatible.
Q3: Will this kit isolate DNA?
Yes, this kit will co-purify some DNA.
Q4: Can DNase I treatment be performed?
Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).
Q5: Is it normal for the Viral RNA (DNA/RNA) Buffer to range in color between clear and yellow?
Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.
Q6: What is the expiration of the Viral RNA Buffer when beta-mercaptoethanol has been added?
The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.
Q7: Why are my columns/plate wells getting clogged?
In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.
Q8: Why am I seeing delayed/no amplification during RT/qPCR?
For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.
“The Quick-RNA Viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection.”
- S.B., Boehringer Ingelheim, Canada
|W1001-10||DNase/RNase-Free Water||10 ml||$20.90|
|W1001-4||DNase/RNase-Free Water||4 ml||$13.20|
|R1200-125||DNA/RNA Shield (2X Concentrate)||125 ml||$241.00|
|R1034-2-48||Viral Wash Buffer (concentrate)||48 ml||$116.60|
|R1034-1-100||Viral RNA Buffer||100 ml||$166.10|
|C2003||Elution Plate||2 Plates||$20.90|
|C2002||Collection Plate||2 Plates||$24.20|
|C2007-2||96-Well Plate Cover Foil||2 Foils||$10.00|
|C2007-4||96-Well Plate Cover Foil||4 Foils||$10.00|
|C2004||Zymo-Spin I-96 Plate||2 Plates||$156.20|