Quick-RNA Viral 96 Kit

R1040 / R1041


Quick-RNA Viral 96 Kit

R1040 / R1041

Cat # Name Size Price Quantity
R1040 Quick-RNA Viral 96 Kit 2 x 96 preps $399.00
+ -
R1041 Quick-RNA Viral 96 Kit 4 x 96 preps $716.00
+ -

Documents


Viral DNA and RNA from all biological fluids

Highlights


  • Quick, high-throughput (96-well spin plate) purification of viral RNA from plasma, serum, CSF, cell culture media, cellular suspensions, urine, blood, saliva, swab, fecal, etc.
  • Omits the use of organic denaturants and proteases.
  • High-quality viral RNA is ready for RT-PCR, sequencing, etc.

Description


The Quick-RNA Viral 96 Kit is a viral RNA purification kit that provides for rapid, large scale isolation of viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield. DNA/RNA Shield ensures nucleic acid stability during sample storage/transport at ambient temperatures. The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the matrix in each well of the spin-plate, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.

Technical Specifications


Equipment Centrifuge with 96-well plate carrier
Purity RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Sample Source Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab and fecal samples
Size Range 50 nt to ~200 kb
Yield Spin plate, 10 µg RNA (binding capacity), ≥10 µl (elution volume)

Reviews


“The Quick-RNA Viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection.”

- S.B., Boehringer Ingelheim, Canada

Kit Components